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V5265

Crystal Violet Solution

1%, aqueous solution

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About This Item

Empirical Formula (Hill Notation):
C25H30ClN3
CAS Number:
548-62-9
Molecular Weight:
407.98
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
329829057
MDL number:
MFCD00011750
Beilstein/REAXYS Number:
3580948
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form

aqueous solution

Quality Level

200

concentration

1%

technique(s)

microbe id | staining: suitable

color

deep violet, purple

Amax

0.36-0.44 at 589-594 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C

InChI

1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1

InChI key

ZXJXZNDDNMQXFV-UHFFFAOYSA-M

General description

Crystal violet, also known as gentian violet or methyl violet, is the N-hexamethylated derivative of pararosaniline. It is a basic, cationic, acidotropic protein dye used in histological and bacteriological staining. It is well-known for its antibacterial and antifungal properties.

It stains the fatty portions of sebaceous sweat a deep purple color. Crystal violet can also be used to enhance bloody fingerprints. This dye is harmful if inhaled, swallowed or absorbed through skin, contact may cause cancer, severe eye irritation in human beings.

Application

Crystal violet solution for use in Brown-Hopps method for Gram-positive and Gram-negative bacterial staining.
  • Crystal violet is commonly used in Gram staining for the classification of bacteria.
  • It has also been used to detect bacterial adherence to biomedical polymers and to stain DNA in mammalian tissues in Giemsa staining.
  • It has been successfully used to develop a counterion-staining method to detect DNA in agarose gel electrophoresis.
  • Its antimicrobial properties have facilitated its use in the treatment of oral candidiasis, skin infections, and methicillin-resistant Staphylococcus aureus.
  • It has been used as a stain in cell proliferation assays, migration assays, and Boyden chamber assay.

Biochem/physiol Actions

Crystal violet is the primary stain in Gram staining. It infiltrates the peptidoglycan-rich cell wall of Gram-positive bacteria, and forms entrapped complexes with Gram′s iodine that retain the purple color post-decolorization. Thus, Gram-positive bacteria can be differentiated from Gram-negative bacteria whose peptidoglycan layer is considerably thinner.
The antimicrobial effects of crystal violet can be attributed to free radical generation and formation of unionized bacterial complexes with the dye. It has also been reported to inhibit protein synthesis and bacterial cell wall formation. It is especially effective against Gram-positive bacteria.

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pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

Hazard Classifications

Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Global Trade Item Number

SKUGTIN
V5265-250ML04061837481116
V5265-500ML04061837481123