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SNC50

mirPremier® microRNA Isolation Kit

1 sufficient for 50 preparations

Synonym(s):

microRNA Isolation Kit

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About This Item

NACRES:
NA.52
UNSPSC Code:
41105501
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Quality Level

200

usage

sufficient for 50 preparations

technique(s)

DNA extraction: suitable

General description

Click here to view the kit protocol
Sigma′s mirPremier microRNA Isolation Kit provides a rapid and efficient method for purifying and enriching miRNAs and other small RNAs from diverse biological sources, including mammalian cell cultures, animal tissues, plant tissues, and microbial cultures, without using hazardous organic extractions. microRNAs (miRNAs) are a class of small RNA molecules, about 21 nucleotides (nt) in length, that regulate gene expression in a variety of manners, including translational repression, mRNA cleavage and deadenylation. In addition, the kit also can be used for isolating total RNA if messenger RNA or other large RNAs are of interest.
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Application

mirPremier® microRNA Isolation Kit has been used to:

  • extract total RNA containing miRNA from grape edible plant derived exosome-like nanoparticles (EPDEN).
  • isolate small RNA from S.sirkka and S. napiecek, and S. arctica.
  • extract miRNA, from frozen rat livers for miRNA analysis.

Features and Benefits

  • Designed to enhance the efficiency of isolating microRNA and other small RNA molecules directly from a wide range of biological sources.
  • Enables fast and efficient extraction and concentration of miRNA in 30 minutes for downstream applications.
  • Can extract high-purity miRNA with no detectable large RNA
  • No dangerous organic extractions are involved.

Legal Information

mirPremier is a registered trademark of Merck KGaA, Darmstadt, Germany

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Kit Components Only

Product No.
Description
  • Binding Solution 2
  • Lysis Solution

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

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Articles

Genomic DNA Purification and Assessment

Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.

Aufreinigung und Qualitätsprüfung von genomischen DNA-Proben

Die Verfügbarkeit von einfachen Verfahren zum Aufreinigen von DNA und RNA hat die Analyse und Charakterisierung des Genoms und der Genexpression in hohem Maß erleichtert. Es besteht eine Anforderung, DNA und RNA schnell und praktisch aus verschiedenen zellulären Quellen, einschließlich Gewebe aus Tier-, Pflanzen- und Bakterienkulturen, zu isolieren.

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Dongxiao Su et al.
Food & function, 8(2), 808-815 (2017-01-26)
Dietary phenolics exhibit hypolipidemic activity by changing lipid metabolism-related microRNA (miRNA) expression. Quercetin 3-O-rutinoside-7-O-α-l-rhamnosidase (quercetin 3-rut-7-rha), rutin and (-)-epicatechin are the main phenolics in lychee (Litchi chinensis Sonn.) pulp. A previous study reported that quercetin 3-rut-7-rha and rutin had hypolipidemic
Interspecies communication between plant and mouse gut host cells through edible plant derived exosome-like nanoparticles.
Mu J
Molecular Nutrition And Food Research, 58(7), 1561-1573 (2014)
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The emergence of multicellular animals was associated with an increase in phenotypic complexity and with the acquisition of spatial cell differentiation and embryonic development. Paradoxically, this phenotypic transition was not paralleled by major changes in the underlying developmental toolkit and
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Global Trade Item Number

SKUGTIN
SNC50-1KT04061837108020