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S2015

SSPE Buffer 20× Concentrate

Name: SSPE Buffer 20× Concentrate
Brand: SIGMA

for Northern and Southern blotting, solution

Synonym(s):

20X SSPE Buffer, SSPE hybridization buffer, Saline-sodium phosphate-EDTA buffer

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About This Item

NACRES:

NA.25

UNSPSC Code:

41105319

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Properties

grade

Molecular Biology

Quality Level

200

sterility

0.2 μm filtered

form

solution

foreign activity

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected

storage temp.

room temp

Description

General description

SSPE Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. This liquid can be used straight or diluted as needed.

Application

Suitable for:

Hybridization buffer for Northern and Southern blots
Washing buffer for microarrays

Features and Benefits

Convenient ready-to-use liquid
Multi-purpose use in hybridizations

Preparation Note

SSPE Buffer 20x contains 0.02M EDTA and 2.98M NaCl in 0.2M phosphate buffer (pH 7.4).

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Protocols

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern und Southern Blot Protokolle und Einführung

Einführung in Northern Blotting und Southern Blotting, bei denen es sich um beliebte Methoden zur Übertragung von Makromolekülen auf Membranen handelt. Dieser Artikel enthält außerdem ein Protokoll für Northern Blotting und ein Protokoll für Southern Blotting.

Genome-wide analysis of barcoded Saccharomyces cerevisiae gene-deletion mutants in pooled cultures.

Pierce SE, et al.

Nature Protocols, 2, 2958-2974 (2007)

Glucocorticoid antagonist RU38486 fails to block acid-induced muscle wasting in vivo or in vitro.

Pickering WP, et al.

Nephrology, Dialysis, and Transplantation, 18, 1475-1484 (2003)