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S1452

MISSION® siRNA Transfection Reagent

Synonym(s):

MISSION® siRNA Transfection Reagent

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About This Item

NACRES:
NA.51
UNSPSC Code:
41106502
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product line

MISSION®

Quality Level

200

form

aqueous solution

shipped in

wet ice

storage temp.

2-8°C

General description

The MISSION® siRNA Transfection Reagent is for the transfection of siRNA to achieve ≥90% silencing efficiency for transient knockdown of eukaryotic gene expression.
Effective gene silencing and reduced off target effects are achieved using a low siRNA concentration (1nM). The siRNA Transfection Reagent is provided as a sterile solution, and is compatible with serum and antibiotics.

Other Notes

Gene silencing assay: An A549 cell line stably expressing firefly luciferase was transfected in 24-well plates with 8.4 ng of 21-mer Luciferase siRNA using 2 μL of the MISSION® siRNA Transfection Reagent. Experiments were performed in triplicate in presence of serum. Cell lysates were assessed for luciferase expression 48 hours post-transfection.

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk

WGK 3

flash_point_f

120.2 °F

flash_point_c

49 °C

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Articles

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

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Transfection Reagent Selection Guide

Browse our convenient transfection reagent selection guide to match the best reagent for your specific cell line and application needs.

Espressione e silenziamento genico

Sigma-Aldrich® Advanced Genomics è il fornitore leader di tecnologie di editing e silenziamento genico tra cui CRISPR, Cas9, RNA guida sintetici (sgRNA) e nucleasi a dita di zinco (ZFN).

Expression et extinction géniques

Sigma-Aldrich® Advanced Genomics est le premier fournisseur de technologies d'édition et d'extinction géniques, dont CRISPR, Cas9, les ARN guides synthétiques (ARNsg) et les nucléases à doigts de zinc (ZFN pour "Zinc Finger Nucleases").

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Global Trade Item Number

SKUGTIN
S1452-100UL04061837743672
S1452-1ML04061836910990