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R4526

Reference Dye for Quantitative PCR

100 ×, solution

Synonym(s):

Reference dye for qPCR, ROX

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About This Item

NACRES:
NA.55
UNSPSC Code:
41106300
Technical Service
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form

solution

Quality Level

300

usage

sufficient for ≥600 reactions

feature

PCR Additives

concentration

100 ×

technique(s)

PCR: suitable

color

red

input

crude DNA

solubility

water: soluble

storage temp.

2-8°C

General description

Sigma′s Reference Dye for quantitative polymerase chain reaction (qPCR) is a proprietary dye for use with real-time PCR. It is used for the normalization of reaction data when using SYBR Green, molecular probes, or dual-labeled probe chemistries for real-time detection. The Reference Dye is supplied as a 100× solution with a maximum excitation and emission of 586 nm and 605 nm, respectively. Instrument settings for ROX reference dye are satisfactory for the measurement of Reference Dye for qPCR.

Application

Reference Dye for Quantitative PCR has been used:
  • in the preparation of mastermix for real time-quantitative polymerase chain reaction (RT-qPCR)
  • as a component of the reaction mixture for detection of Clostridium difficile by quantitative polymerase chain reaction (qPCR)
  • for analyzing the degree of cellular DNA contamination by qPCR

Other Notes

Reference Dye for Quantitative PCR is forR&D use only. Not for drug, household, or other uses.

pictograms

Exclamation markEnvironment
GHS07,GHS09

signalword

Warning

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

ppe

Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
016K61711
077K6177
126K60521
067K6116
126K6052

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Protocols

Protocollo universale SYBR Green qPCR

Il nostro protocollo SYBR Green qPCR è un metodo progettato per la quantificazione accurata dell'espressione genica e delle reazioni RT-PCR.

SYBR® Green JumpStart™ Taq ReadyMix™

Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.

Universal SYBR Green qPCR Protocol

Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions

View All Protocols

Articles

Sample Prep & Gel Electrophoresis Troubleshooting

Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.

Probenvorbereitung & Fehlersuche in der Gelelektrophorese

Mögliche Ursachen und Abhilfemaßnahmen für Probleme, die bei der Vorbereitung von Proben für SDS-PAGE (Natriumdodecylsulfat-Polyacrylamid-Gelelektrophorese) auftreten, und Optimierung der Elektrophoresebedingungen.

Increased environmental sample area and recovery of Clostridium difficile spores from hospital surfaces by quantitative PCR and enrichment culture
Brown KA, et al.
Infection Control and Hospital Epidemiology : The Official Journal of the Society of Hospital Epidemiologists of America, 39(8), 917-923 (2018)
Partial deletion of rng (RNase G)-enhanced homoethanol fermentation of xylose by the non-transgenic Escherichia coli RM10
Manow R, et al.
Journal of Industrial Microbiology & Biotechnology, 39(7), 977-985 (2012)
Sambrook, J. et al.
Molecular Cloning: A Laboratory Manual, 3rd (2000)
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Global Trade Item Number

SKUGTIN
R4526-.3ML04061836695354
R4526-20RXN04061832933283
R4526-5ML04061837693939