Key Documents

View All Documentation

R0883

RPMI-1640 Medium

Name: RPMI-1640 Medium
Brand: SIGMA

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Roswell Park Memorial Institute 1640 medium

Select a Size

Change View

About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

MDL number:

MFCD00217820

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

Product Name

RPMI-1640 Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: no
phenol red: yes
L-glutamine: no
NaHCO₃: yes
HEPES: no

shipped in

ambient

storage temp.

2-8°C

Description

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Application

RPMI-1640 Medium has been used:

to culture PC-3 prostate cell line and Jurkat cells
to maintain a triple-negative MDA-MB-231 and a luminal type MCF7 human Caucasian breast adenocarcinoma cell line
to cultivate circulating tumor cells (CTCs)

Preparation Note

Supplement with 0.3 g/L L-glutamine.

Still not finding the right product?

Explore all of our products under RPMI-1640 Medium

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Protocols

Protocollo del saggio di proliferazione e vitalità cellulare con MTT

Protocollo del saggio di proliferazione e vitalità cellulare e citotossicità con MTT. Istruzioni per la preparazione dei reagenti per il saggio con MTT con esempi di applicazione.

MTT Assay Protocol for Cell Viability and Proliferation

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

Cell Viability and Proliferation XTT Assay Protocol Guide

Perform colorimetric assays for nonradioactive quantification of cellular proliferation, viability, and cytotoxicity for adherent or suspension cells cultured in 96-well microplates.

View All Protocols

MUC1 antigen-specific CD8 T lymphocytes targeting MCF7 and MDA-MB-231 human breast adenocarcinoma cell lines

Parsonidis P, et al.

Journal of Cancer Therapy, 10(07), 495-495 (2019)

Intravenous Iron Sucrose Therapy in Iron Deficiency Anemia in Antenatal and Postnatal Patients.

M Mathur et al.

JNMA; journal of the Nepal Medical Association, 53(198), 108-112 (2016-03-20)

Dermatophytosis is a common superficial fungal infection of the skin, hair and nails caused by Trichophyton, Microsporum and Epidermophyton dermatophytic species. Identification of causative dermatophytic species tend to vary with time and place and antifungal sensitivity is of epidemiological concern

Filaricidal properties of Lantana camara and Tamarindus indica extracts, and Lantadene A from L. camara against Onchocerca ochengi and Loa loa.

Adela Ngwewondo et al.

PLoS neglected tropical diseases, 12(6), e0006565-e0006565 (2018-06-14)

Ivermectin is the only drug currently recommended for the treatment of onchocerciasis, the second leading infectious cause of blindness in the world. This drug kills only the first stage larvae-microfilariae (mf) of Onchocerca volvulus and is to be used cautiously

View All Related Papers

Global Trade Item Number

SKU	GTIN
R0883-100ML	04061836694036
R0883-6X1L	04061836694067
R0883-6X500ML	04061836694074
R0883-10L	04061837341434
R0883-1L	04061838259943
R0883-20L	04061837341441
R0883-24X500ML	04061836694043
R0883-500ML	04061836694050