Skip to Content
Merck

Key Documents

View All Documentation

P7828

Percoll®

suitable for plant cell culture

Synonym(s):

density gradient medium

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

UNSPSC Code:
10171502
NACRES:
NA.72
MDL number:
MFCD00131830
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

sterility

aseptically filled

Quality Level

200

form

colloidal (Colloidal solution of silica coated with polyvinylpyrrolidone (PVP))

technique(s)

cell culture | plant: suitable

pH

8.9 (20 °C)

application(s)

agriculture

storage temp.

2-8°C

Application

Percoll® has been used in the density gradient centrifugation isolation of intact chloroplasts from green alga, decidual stromal cells and distinct populations of rooster sperm.
Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used in cell separation and organelle isolation.
Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used to establish non-toxic low viscosity, low osmolarity density gradients that can be used to isolate cells, organelles and viruses. It has been used to separate inner and outer membrane vesicles from E. coli. Percoll is isosmotic throughout the gradient, non-toxic, and is easily removed from the purified materials.
Percoll is used in plant research for protoplast and organelle (chloroplast, mitochondria) separation.

Legal Information

Percoll is a registered trademark of Cytiva

Still not finding the right product?

Explore all of our products under Percoll®

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
116K8901
068K8745
068K8746
038K0739
037K0668

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Protocols

How to Make and Use Percoll Gradients

To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.

Herstellung und Verwendung von Percoll-Gradienten

Zur Herstellung eines Percoll-Gradienten muss die Osmolalität von Percoll mit Kochsalzlösung oder Zellkulturmedium eingestellt werden, damit Percoll mit physiologischen Salzlösungen isotonisch wird.

Sperm Collection of Differential Quality Using Density Gradient Centrifugation
Ahammad MU, et al.
Journal of Visualized Experiments, 145(141), e58833-e58833 (2018)
Guangkun Yin et al.
Plant & cell physiology, 50(7), 1305-1318 (2009-06-13)
The development of mitochondria during seed germination is essential for plant growth. However, the developmental process is still poorly understood. Temperature plays a key role in soybean germination, and in this study we characterized the mitochondrial ultrastructure and proteome after
A rapid method for chloroplast isolation from the green alga Chlamydomonas reinhardtii
Mason CB, et al.
Nature Protocols, 1(5), 2227-2227 (2006)
View All Related Papers

Global Trade Item Number

SKUGTIN
P7828-100ML04061834398219
P7828-1L04061834398226
P7828-25ML04061834398233
P7828-500ML04061834398240