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P7582

Amino-terminal FLAG-BAP™ Fusion Protein

Synonym(s):

Amino-terminal Fusion Protein, BAP Fusion Protein, FLAG-BAP Fusion Protein

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
MFCD00130921
Form:
liquid
Mol wt:
~49 kDa
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form

liquid

Quality Level

200

mol wt

~49 kDa

shipped in

dry ice

storage temp.

−20°C

General description

Amino-terminal FLAG-BAP™ Fusion Protein is a 467 amino acid N-terminal FLAG fusion protein of E. coli bacterial alkaline phosphatase (BAP) with a calculated molecular mass of 49.3kDa. The N-terminal FLAG-BAP Fusion Protein migrates as a 45−55kDa band by SDS-PAGE depending on electrophoresis conditions. FLAG-BAP™ Fusion Protein has biotechnological applications and is used in the screening of recombinant fusion proteins.
The Amino-terminal FLAG-BAP Fusion Protein is a control prorein used to confirm the funtional integrity of the anti-FLAG M1 and M2 monoclonal antibodies in applications such as western blotting, immunoprecipitation, ELISA, electron microscopy, and FACS.

Application

Amino-terminal FLAG-BAP™ Fusion Protein has been used as a standard in enzyme-linked immunosorbent assay (ELISA) for osteopontin quantification and in western blotting based quantification of Flag-Wnt8 and Drosophila melanogaster transcription factor interactome.
Learn more product details in our FLAG® application portal.

Physical form

Protein is supplied in 10 mM Tris, 120 mM NaCl,
0.05 mM ZnCl2 in 50% glycerol, pH 8.0.

Other Notes

Control protein

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
045K61231
098K6107
087K6125
081K9130
105K6255

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Protocols

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

Immunpräzipitation von FLAG-Fusionsproteinen mit Affinitätsgelen monoklonaler Antikörper

Protokoll für die Immunpräzipitation (IP) von FLAG-Fusionsproteinen unter Einsatz des monoklonalen M2-Antikörper-Affinitätsgels (4 % Agarose)

Articles

Anti-FLAG® M2 Magnetic Beads

Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.

Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

Anti-FLAG® M2 Magnetperlen

Vergleich von Elutionstechniken für die Proteinaufreinigung von FLAG® Tag Proteinen im kleinen Maßstab mittels anti-FLAG® M2 Magnetperlen.

Related Content

Data Sheet - P7582
Expression des protéines

Technologies d'expression de protéines destinées à différents systèmes d'expression répondant aux besoins de la recherche et de la production d'agents thérapeutiques et de vaccins.

Purification des protéines

Techniques, réactifs et protocoles permettant de purifier les protéines recombinantes par différentes méthodes telles que la chromatographie d'échange d'ions, la chromatographie d'exclusion stérique ou la chromatographie d'affinité.

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Eroshkin FM, et al.
Scientific Reports, 6, 23049-23049 (2016)
Exploring the separation power of mixed-modal resins for purification of recombinant osteopontin from clarified Escherichia coli lysates
Guo S, et al.
Biotechnology Progress, 35(1), e2722-e2722 (2019)
A Comprehensive Drosophila melanogaster Transcription Factor Interactome
Shokri L, et al.
Cell Reports, 27(3), 955-970 (2019)
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Global Trade Item Number

SKUGTIN
P7582-100UG04061834395362
P7582-25UG04061833266045
P7582-40UG04061833266052
P7582-50UG04061833266069