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M3148

2-Mercaptoethanol

Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

Synonym(s):

β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

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About This Item

Linear Formula:
HSCH2CH2OH
CAS Number:
60-24-2
Molecular Weight:
78.13
UNSPSC Code:
12161504
NACRES:
NA.31
PubChem Substance ID:
24896800
EC Number:
200-464-6
Beilstein/REAXYS Number:
773648
MDL number:
MFCD00004890
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grade

Molecular Biology

vapor density

2.69 (vs air)

vapor pressure

1 mmHg ( 20 °C)

product line

BioReagent

assay

99% (GC/titration)

form

liquid

expl. lim.

18 %

reaction suitability

reagent type: reductant

concentration

14.3 M (pure liquid)

technique(s)

cell culture | mammalian: suitable, electrophoresis: suitable

refractive index

n20/D 1.500 (lit.)

pH

4.5-6 (20 °C, 500 g/L)

bp

157 °C (lit.)

solubility

H2O: soluble 1 mL/mL

density

1.114 g/mL at 25 °C (lit.)

foreign activity

DNase, RNase, protease, none detected

storage temp.

2-8°C

SMILES string

OCCS

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

Application

Reducing agent
β-mercaptoethanol or 2-mercaptoethanol has been used:
  • as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture DT40 cells (chicken B cell line)
  • to lyse NuLi-1 (Normal Lung, University of Iowa-1) cells
  • to trypsinize mouse embryonic stem cells
BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

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Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

target_organs

Liver,Heart

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

flash_point_f

165.2 °F - closed cup

flash_point_c

74 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
125K0165
118K0106
088K0012
108K0032
058K0020

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This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

MTT Assay Protocol for Cell Viability and Proliferation

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

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A subset of platinum-containing chemotherapeutic agents kills cells by inducing ribosome biogenesis stress
Peter M Bruno
Nature Medicine, 23 (2017)
Reduced Innate Immune Response to a Staphylococcus aureus Small Colony Variant Compared to Its Wild-Type Parent Strain
Judy J
Frontiers in Cellular and Infection Microbiology, 6, 187-187 (2016)
Kotaro Fujii et al.
Nature communications, 8, 14443-14443 (2017-02-15)
The degree and dynamics of translational control during mammalian development remain poorly understood. Here we monitored translation of the mammalian genome as cells become specified and organize into tissues in vivo. This identified unexpected and pervasive translational regulation of most
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Global Trade Item Number

SKUGTIN
M3148-.15ML04061838080097
M3148-.9ML04061838243348
M3148-25ML04061838258496
M3148-2ML04061838243355
M3148-100ML04061838258472
M3148-250ML04061838258489
M3148-500ML04061834046424