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A9357

Anti-alpha-Actin Antibody, Cardiac Muscle (ACTC1) Antibody

mouse monoclonal, AC1-20.4.2

Synonym(s):

Anti-ACTC, Anti-ACTC1, Anti-Actin alpha cardiac muscle 1, Anti-CMD1R, Anti-CMH11

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
Conjugate:
unconjugated
Clone:
AC1-20.4.2, monoclonal
Application:
ELISA (i), ICC, IHC (p), WB
Citations:
21
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Product Name

Anti-Actin, Cardiac antibody, Mouse monoclonal, clone AC1-20.4.2, purified from hybridoma cell culture

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AC1-20.4.2, monoclonal

form

buffered aqueous solution

mol wt

antigen ~42 kDa

species reactivity

rat, human, bovine, chicken, mouse

concentration

~1.0 mg/mL

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using digested rat heart tissue, indirect ELISA: suitable, western blot: suitable

isotype

IgG1

UniProt accession no.

P68032

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACTC1(70)
mouse ... Actc1(11464)
rat ... Actc1(29275)

General description

Actins are cytoskeletal proteins that are widely expressed in eukaryotes. These proteins primarily regulate various cellular functions such as muscle contraction, mRNA synthesis and apoptosis among many others. ACTC1 is the predominant actin isoform form in adult cardiac tissues and alterations in this isoform have been associated with hypertrophic cardiomyopathy and congenital heart disease . Monoclonal Anti-Actin, Cardiac antibody is specific for human, bovine, chicken, rat, and mouse cardiac α actin.

Immunogen

synthetic NH2-terminal decapeptide of cardiac isoform of actin.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
Monoclonal Anti-Actin, Cardiac antibody is suitable for use in immunocytochemistry, immunoblot (approx. 42 kDa), indirect ELISA, and western blot. The antibody may also be used in immunohistochemistry (using formalin-fixed, paraffin-embedded sections, or using a conc. of 10-20 μg/mL in digested rat heart tissue).
Protein samples isolated from cardiac sections or cardiac myocytes were subjected to western blot using monoclonal mouse anti-actin antibody as the primary at a dilution of 1:2000.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

wgk

WGK 1

Storage Class

12 - Non Combustible Liquids

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Certificates of Analysis (COA)

Lot/Batch Number
098K4771

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Related Content

Iel-Yong Sung et al.
International journal of medical sciences, 13(11), 841-852 (2016-11-24)
The purpose of the present study was to investigate the in vitro cardiomyogenic differentiation potential of human dental follicle-derived stem cells (DFCs) under the influence of suberoylanilide hydroxamic acid (SAHA), a member of the histone deacetylase inhibitor family, and analyze
W W Franke et al.
Differentiation; research in biological diversity, 60(4), 245-250 (1996-07-01)
We describe three murine monoclonal antibodies (mAbs) raised against a synthetic decapeptide representing the aminoterminal sequence of the cardiac/ fetal isoform of sarcomeric alpha-actin. When used for immunoblotting or histological immunolocalization, these mAbs distinguish cardiac/fetal alpha-actin from skeletal muscle alpha-actin
Mirco Müller et al.
Cellular and molecular life sciences : CMLS, 69(20), 3457-3479 (2012-05-31)
Inherited cardiomyopathies are caused by point mutations in sarcomeric gene products, including α-cardiac muscle actin (ACTC1). We examined the biochemical and cell biological properties of the α-cardiac actin mutations Y166C and M305L identified in hypertrophic cardiomyopathy (HCM). Untagged wild-type (WT)
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Global Trade Item Number

SKUGTIN
A9357-100UL04061837718984
A9357-200UL04061833402382