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Enhanced Avian First Strand Synthesis Kit

Components for cDNA synthesis with enhanced AMV reverse transcriptase

Synonym(s):

Reverse Transcriptase cDNA synthesis kit, eAMV-RT

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About This Item

NACRES:
NA.55
UNSPSC Code:
12352200
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Quality Level

200

usage

sufficient for 50 reactions,  kit sufficient for 50 reactions

feature

dNTPs included, hotstart: no

technique(s)

RT-PCR: suitable, cDNA synthesis: suitable

color

colorless

input

purified RNA

shipped in

dry ice

storage temp.

−20°C

General description

Enhanced Avian First Strand Synthesis Kit utilizes a highly purified avian myeloblastosis virus reverse transcriptase (eAMV-RT) that offers superior performance in comparison to standard AMV-RT or standard Moloney murine leukemia virus reverse transcriptase (MMLV-RT). This exceptionally robust eAMV-RT has an enhanced ability to transcribe through difficult secondary structure at elevated temperatures (up to 65 °C) making it the ideal enzyme for producing high quality full-length cDNA from total RNA or poly(A)+ RNA.
Sigma′s Enhanced Avian RT First Strand Synthesis Kit also provides random nonamers (9-mers) and anchored oligo (dT)23 primers since gene-specific primers may not always be useful or possible. The anchored oligo has 23 thymidine residues and one G, C, or A residue (the anchor). The anchor ensures that the oligo (dT) primer binds at the very beginning of the message and that there is not a long region of the unusable sequence. With this kit, a dependable cDNA is generated that can be used for various downstream applications, including PCR.

Application

Enhanced Avian First Strand Synthesis Kit has been used for cDNA synthesis, which is intended for use in a real-time reverse transcription-polymerase chain reaction (RT-PCR).

Other Notes

One unit incorporates one nanomole of TMP into TCA-precipitable material in 10 minutes using polyadenylic acid as template and oligo(dT)12-18 as primer.

Legal Information

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

Kit Components Only

Product No.
Description
  • Enhanced Avian Reverse Transcriptase 1000 U

Kit Components Also Available Separately

Product No.
Description
SDS & Pricing
  • Deoxynucleotide mix 50 μL
    SDS
  • O4387
    Anchored oligo (dT)23 100 μL
    SDS
  • R7647
    Random nonamers 100 μL
    SDS
  • O4387
    Ribonuclease inhibitor 50 μL
    SDS
  • W1754
    PCR grade water 1.5 mL
    SDS

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

signalword

Danger

hcodes

EUH430

Hazard Classifications

ED ENV 1

wgk

WGK 3

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
026K6847
025K6066
114K6029
094K6022
103K9070

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Protocols

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

Articles

Reverse Transcription

Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.

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RT-qPCR – Quantitative Reverse Transcription PCR

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RT-qPCR (PCR quantitative après transcription inverse)

La RT-qPCR, ou PCR quantitative après transcription inverse, combine les effets de la transcription inverse et de la PCR quantitative, ou PCR en temps réel, pour amplifier et détecter des cibles spécifiques. La RT-qPCR trouve de multiples applications, notamment dans la quantification du niveau d'expression génique, la validation de l'interférence par ARN ou la détection d'agents pathogènes comme les virus.

Applicazioni della PCR

La PCR (reazione a catena della polimerasi) è una tecnica che consente di amplificare le molecole di acido nucleico comunemente usata in molte applicazioni fra cui RT-PCR, hot start PCR, end point PCR ed altre.

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Global Trade Item Number

SKUGTIN
STR1-1KT04061835574988