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D4545

Taq DNA Polymerase from Thermus aquaticus

Name: Taq DNA Polymerase from <I>Thermus aquaticus</I>
Brand: SIGMA

with 10× PCR reaction buffer without MgCl₂

Synonym(s):

Taq polymerase, Taq polymerase enzyme

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About This Item

CAS Number:

9012-90-2

UNSPSC Code:

12352204

NACRES:

NA.55

EC Number:

2.7.7.7 (BRENDA, IUBMB)

MDL number:

MFCD00166026

Biological source:

enzyme from bacterial (Thermus Aquaticus)

Recombinant:

expressed in E. coli

Concentration:

5 units/μL

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Properties

biological source

enzyme from bacterial (Thermus Aquaticus)

Quality Level

200

recombinant

expressed in E. coli

form

liquid

usage

sufficient for 1500 reactions, sufficient for 250 reactions, sufficient for 50 reactions, sufficient for 5000 reactions

feature

dNTPs included: no, hotstart: no, Standard PCR

concentration

5 units/μL

technique(s)

PCR: suitable

color

colorless

input

purified DNA

suitability

suitable for PCR and automated sequencing reactions

application(s)

agriculture

shipped in

wet ice

storage temp.

−20°C

Description

General description

Taq polymerase is a thermostable DNA polymerase named after the thermophilic bacterium Thermus aquaticus. A stable deoxyribonucleic acid (DNA) polymerase (with a temperature optimum of 80°C) has been purified from the extreme thermophile Thermus aquaticus. The enzyme is free from phosphomonoesterase, phosphodiesterase and single-stranded exonuclease activities. Maximal activity of the enzyme needs all four deoxyribonucleotides and activated calf thymus DNA.

Application

Taq DNA Polymerase from Thermus aquaticus has been used:

in the process of DNA extraction (during gene amplification and sequencing)
in genotyping
in polymerase chain reaction (PCR) to study the constitutive production of epithelial neutrophil activating peptide 78 (ENA-78) and interleukin-8 (IL-8)
for amplification of RNA from primary endothelial cells by conventional PCR

Biochem/physiol Actions

Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. It displays both 5′ to 3′ polymerase and exonuclease activities.

Features and Benefits

MgCl₂ provided in a separate tube to allow MgCl₂ optimization
Can withstand repeated heating to 95 °C without significant loss of activity

Packaging

Taq DNA Polymerase with 10× reaction buffer without MgCl₂. Includes a separate tube of 25 mM MgCl₂

Taq DNA polymerase comes with the choice of an optimized 10× reaction buffer including MgCl₂ (D1806) or a 10× reaction buffer without MgCl₂ plus a separate tube of MgCl₂ for titration (D4545). The latter option may be necessary to determine optimal conditions for amplification.

Other Notes

One unit will incorporate 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Taq DNA Polymerase is a specialized thermostable enzyme isolated from the thermophilic bacterium Thermus aquaticus. The recombinant form of this enzyme is expressed in E. coli. This 94 kDa protein shows no detectable levels of contaminating endonucleases or exonucleases by SDS-PAGE. It has both 5′→3′ polymerase and exonuclease activity.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

Hazard Classifications

Aquatic Chronic 3

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

107H9020

107H9019

057H6661

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Protocols

Protocollo PCR standard

Imparate le fasi del protocollo PCR standard e rivedete gli elenchi dei reagenti o i parametri di ciclaggio. Questo metodo per l'amplificazione PCR di routine del DNA utilizza la Taq DNA polimerasi standard.

Hot Start dNTP Protocol

Hot start dNTP protocol enhances specificity in PCR by blocking DNA polymerase nucleotide incorporation during PCR.

dNTP Hot Start PCR Protocol

Hot Start dNTPs block DNA polymerase until heat activation, enhancing PCR specificity.

View All Protocols

Articles

Introduction & Historical Timelines

Explore PCR's history, from discovery to Nobel Prize. Discover real-time PCR (qPCR) and digital PCR developments.

Polymerase Chain Reaction

The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension

Réaction en chaîne par polymérase (PCR)

La réaction en chaîne par polymérase (PCR) est l'une des techniques les plus couramment utilisées en biologie moléculaire. Le procédé de PCR implique trois étapes principales : la dénaturation, l'hybridation des amorces et l'élongation

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Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus.

A Chien et al.

Journal of bacteriology, 127(3), 1550-1557 (1976-09-01)

A stable deoxyribonucleic acid (DNA) polymerase (EC 2.7.7.7) with a temperature optimum of 80 degrees C has been purified from the extreme thermophile Thermus aquaticus. The enzyme is free from phosphomonoesterase, phosphodiesterase and single-stranded exonuclease activities. Maximal activity of the

Basal and cytokine-stimulated production of epithelial neutrophil activating peptide-78 (ENA-78) and interleukin-8 (IL-8) by cultured human endometrial epithelial and stromal cells.

Nick A Bersinger et al.

Fertility and sterility, 89(5 Suppl), 1530-1536 (2007-09-01)

To analyze the constitutive production of epithelial neutrophil activating peptide 78 (ENA-78) and interleukin-8 (IL-8) by epithelial cells and the response of these cells to cytokine stimulation. In vitro study using eutopic endometrial tissue. University hospital. Cycling women undergoing laparoscopy

Hdac6 knock-out increases tubulin acetylation but does not modify disease progression in the R6/2 mouse model of Huntington's disease.

Anna Bobrowska et al.

PloS one, 6(6), e20696-e20696 (2011-06-17)

Huntington's disease (HD) is a progressive neurodegenerative disorder for which there is no effective disease modifying treatment. Following-on from studies in HD animal models, histone deacetylase (HDAC) inhibition has emerged as an attractive therapeutic option. In parallel, several reports have

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Global Trade Item Number

SKU	GTIN
D4545-1.5KU	04061835512423
D4545-20X250UN	04061835572670
D4545-250UN	04061835572687
D4545-50UN	04061835572694
D4545-5KU	04061835572700