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11191

Histopaque®-1119

sterile-filtered, density: 1.119

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About This Item

NACRES:
NA.75
UNSPSC Code:
12352207
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sterility

sterile-filtered

Quality Level

400

form

liquid

shelf life

Expiry date on the label.

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

density

1.119

application(s)

hematology
histology

storage temp.

2-8°C

General description

Histopaque®-1119 is a sterile, endotoxin tested, ready-to-use medium. This solution consists of polysucrose and sodium diatrizoate, adjusted to a relative density of 1.119 g/mL and pH 8.8–9.0.

Application

Histopaque®-1119 is suitable for use in conjunction with Histopaque-1077 as it facilitates the rapid recovery of viable mononuclear cells and granulocytes from small volumes of whole blood or bone marrow

Biochem/physiol Actions

The process involves the formation of a double gradient on layering an equal volume of Histopaque-1077 over Histopaque-1119. Anticoagulated whole blood is carefully layered onto the upper Histopaque-1077 medium. Upon centrifugation, the erythrocytes are aggregated by polysucrose and thus rapidly sediment. Granulocytes are found at the lower Histopaque-1077/1119 interface; whereas, lymphocytes and other mononuclear cells are found at the upper plasma/Histopaque-1077 interface. Most extraneous platelets found in the upper interface are removed by low-speed centrifugation during washing steps. Erythrocyte contamination is negligible.

Features and Benefits

Histopaque®-1119 offers numerous advantages, such as:
  • Optimal recovery of viable cells
  • Permits selective separation of blood cell types
  • Prevents cell distortion
  • Preserves cell viability
  • Minimal extraneous cell interference
  • Reproducible performance from batch to batch
  • Sterile-filtered and endotoxin-tested
  • Stable at 2-8°C for at least 2 years when protected from light

Legal Information

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Corrosion
GHS05

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Storage Class

10 - Combustible liquids

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Certificates of Analysis (COA)

Lot/Batch Number
126K6002
035K6200
115K6030

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Protocols

Ficoll® 400 for Gradient Centrifugation

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

Histopaque® Density Gradient Media Troubleshooting Guide

This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.

Ficoll® 400 für die Gradientenzentrifugation

Ficoll®-400 ist ein hochverzweigtes Polymer, das durch Copolymerisation von Saccharose und Epichlorhydrin gebildet wird. Ficoll® 400 ist vollständig nicht-ionisch. Aufgrund der großen Anzahl an Hydroxylgruppen ist Ficoll® 400 sehr hydrophil und extrem wasserlöslich.

Articles

Centrifugation Basics

Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.

Darin Quach et al.
Journal of bacteriology, 191(7), 2023-2032 (2008-12-31)
Group B Streptococcus (GBS) is major cause of invasive disease in newborn infants and the leading cause of neonatal meningitis. To gain access to the central nervous system (CNS), GBS must not only subvert host defenses in the bloodstream but
Atsushi Masuda et al.
Nutrients, 11(5) (2019-05-15)
Long-term exposure to a high starch, low-protein diet (HSTD) induces body weight gain and hyperinsulinemia concomitantly with an increase in β-cell mass (BCM) and pancreatic islets number in mice; however, the effect of short-term exposure to HSTD on BCM and
Ulrich Flögel et al.
Circulation, 118(2), 140-148 (2008-06-25)
In this study, we developed and validated a new approach for in vivo visualization of inflammatory processes by magnetic resonance imaging using biochemically inert nanoemulsions of perfluorocarbons (PFCs). Local inflammation was provoked in 2 separate murine models of acute cardiac
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Global Trade Item Number

SKUGTIN
251429-10G04061838138606
251429-50G04061838138613
11191-6X100ML04061838260659
11191-100ML04061838699305