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About This Item
usage
sufficient for 100 reactions, sufficient for 500 reactions, sufficient for 5000 reactions
feature
dNTPs included, hotstart
manufacturer/tradename
Roche
packaging
pkg of 100 x 20 μL reactions (07260288190), pkg of 500 x 20 μL reactions (07260296190), pkg of 5000 x 20 μL reactions (07249926190)
technique(s)
RT-qPCR: suitable, qPCR: suitable
input
purified DNA
detection method
probe-based
General description
The EagleTaq Universal Master Mix (ROX) is a ready-to-use, 2× concentrated PCR master mix that contains all the reagents (except primers, probes, and template) needed for performing quantitative, real-time PCR hydrolysis probe reactions. It contains a special ROX reference dye which makes it suitable for all real-time instruments on which a ROX reference dye is needed for quantitative analysis.
The EagleTaq Universal Master Mix (ROX) contains dUTP so that it may be used with Uracil-DNA Glycosylase (UNG) to prevent false positives arising from carryover contamination.
The hot start properties allow reaction setup at ambient temperature.
With this robust reagent, any PCR protocol optimization is minimized
Application
Features and Benefits
- The EagleTaq Universal Master Mix (ROX) contains a special ROX reference dye which makes it suitable for all real-time instruments on which a ROX reference dye is needed for quantitative analysis.
- It contains dUTP so that it may be used with Uracil-DNA Glycosylase (UNG) to prevent false positives arising from carryover contamination.
- The hot start properties allow reaction setup at ambient temperature.
- With this robust reagent, any PCR protocol optimization is minimized.
Other Notes
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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Articles
PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.
Ein PCR-Mastermix ist eine Charge von PCR- oder RT-PCR-Reagenzien, die auf viele PCR-Reaktionsröhrchen aufgeteilt werden kann. Es enthält in der Regel DNA-Polymerase, dNTP, MgCl2 und Puffer. Erstellen Sie Ihren eigenen Mastermix oder wählen Sie einen kommerziellen Mastermix aus.
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La réaction en chaîne par polymérase (PCR pour "Polymerase Chain Reaction") est une technique d'amplification des molécules d'acide nucléique qui est couramment employée dans de nombreuses applications, incluant la RT-PCR, la PCR à démarrage à chaud, la PCR en point final, et bien d'autres.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 7260288190 | 04061835343515 |
| 7249926190 | 04061835343508 |
| 7260296190 | 04061835343522 |