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CELLPRO-RO

Roche

Cell Proliferation Reagent WST-1

suitable for protein quantification, suitable for cell analysis, detection, solution

Synonym(s):

cell proliferation reagent, wst-1

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About This Item

NACRES:
NA.21
UNSPSC Code:
12352200
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form

solution

usage

sufficient for ≤2,500 tests (11644807001), sufficient for ≤800 tests (05015944001)

packaging

bottle of 25 mL (11644807001), bottle of 8 mL (05015944001)

manufacturer/tradename

Roche

storage condition

protect from light

technique(s)

protein quantification: suitable

optimum pH

8.0(for physiological conditions our product is buffered pH 7.3)

λmax

440-480 nm

application(s)

cell analysis
detection

detection method

colorimetric

storage temp.

−20°C

General description

Contents
Ready-to-use solution, containing WST-1 and an electron coupling reagent.
Cellular proliferation requires the replication of genomic DNA. Thus, monitoring DNA synthesis is an indirect parameter of cell proliferation, as well as being suitable for the study of the regulation of DNA synthesis. [3H]-thymidine has traditionally been used to label the DNA of replicating (cycling) cells. To circumvent the disadvantages associated with the use of the [3H]-thymidine, nonradioactive alternatives based on 5-bromo-2′-deoxyuridine (BrdU) have been developed. The use of chemiluminescence technology provides enhanced sensitivity and a broad measurement range.
Colorimetric assay (WST-1 based) for the nonradioactive quantification of cell proliferation, cell viability, and cytotoxicity.

Application

The Cell Proliferation Reagent WST-1 is used for the nonradioactive, spectrophotometric quantification of cell proliferation and viability in cell populations using the 96-well-plate format. Measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients.
  • Analysis of cytotoxic and cytostatic compounds, such as anti-cancer drugs and other pharmaceutical compounds
  • Assessment of growth inhibitory antibodies and physiological mediators

Biochem/physiol Actions

The stable tetrazolium salt WST-1 is cleaved to a soluble formazan by a complex cellular mechanism that occurs primarily at the cell surface. This bioreduction is largely dependent on the glycolytic production of NAD(P)H in viable cells. Therefore, the amount of formazan dye formed directly correlates to the number of metabolically active cells in the culture.
Cells grown in a 96-well tissue culture plate are incubated with the WST-1 reagent for 0.5 - 4 hours. After this incubation period, the formazan dye formed is quantitated with a scanning multi-well spectrophotometer (ELISA reader). The measured absorbance directly correlates to the number of viable cells.
Cell proliferation and viability assays are of particular importance for routine applications in cell biology. Tetrazolium salts (e.g., MTT, XTT, WST-1) are particularly useful for this type of analysis. Tetrazolium salts are cleaved to formazan by the succinate-tetrazolium reductase system (EC 1.3.99.1) which belongs to the respiratory chain of the mitochondria, and is only active in metabolically intact cells.

Features and Benefits

  • Convenient: Benefit from a ready-to-use reagent.
  • Safe and Easy: Eliminate radioactive isotopes, washing steps, and additional reagents.
  • Accurate: The absorbance obtained strongly correlates to the cell number.
  • Sensitive: Detect low cell numbers.
  • Fast: Process a large number of samples using a multi-well ELISA reader.

Preparation Note

Working concentration: For best results, add 10 μl/well Cell Proliferation Reagent WST-1 to the cells already cultured in 100 μl/well (1:10 final dilution).
Using the 100 μl/well cell culture volume, one vial will be sufficient to perform 2500 tests (25 microplates).
Note: If the cells are cultured in 200 μl/well, add 20 μl/well Cell Proliferation Reagent WST-1.
Storage conditions (working solution): 2 to 8 °C
Note: When precipitates or turbidity are observed upon thawing, warm up the solution to 37 °C for 2 to 10 minutes and agitate to dissolve the precipitates.Centrifugation is not recommended because the working concentration would decrease. After being dissolved, the WST-1 reagent can be used without any limitations. Please store as follows:
Once thawed, store at 2 to 8 °C, protected from light, for up to four weeks. However please note that the solution may become viscous. If so, warm up the solution to 37 °C for 2 to 10 minutes as described above.

Long storage
For long storage, aliquots of WST-1 can be stored in plastic tubes at -15 to -25 °C until the expiry date.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

No data available

flash_point_c

No data available

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Certificates of Analysis (COA)

Lot/Batch Number
20759400
19615100
14606400
12269700
10569200

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Protocols

WST-1 Assay Protocol for Cell Viability

WST-1 assay protocol for cell viability and cytotoxicity measurements with preparation instructions, applications, FAQs, and troubleshooting.

Protokollleitfaden: WST-1-Assay für die Zellproliferation und Viabilität

WST-1-Assayprotokoll für die Messung von Zellviabilität, Zellproliferation, Aktivierung und Zytotoxizität. Anwendungshinweise für die WST-1 Reagenzvorbereitung und Beispiele für Anwendungen. Häufig gestellte Fragen und Anleitung für die Fehlersuche für den WST-1 Assay

Guide technique : test de prolifération et de viabilité cellulaires au WST-1

Protocole du test au WST-1 pour la mesure de la viabilité, de la prolifération, de l'activation et de la toxicité cellulaires. Instructions de préparation du réactif WST-1 et exemples d'applications. Foire aux questions et guide de résolution des problèmes liés au test au WST-1.

Articles

Saggi di vitalità e proliferazione cellulare

Saggi basati su cellule per la proliferazione cellulare (BrdU, MTT, WST1), la vitalità cellulare e gli esperimenti di citotossicità per applicazioni nella ricerca sul cancro, sulle neuroscienze e sulle cellule staminali.

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Assays zur Zellviabilität und -proliferation

Zellbasierte Assays für Zellproliferation (BrdU, MTT, WST1), Zelllebensfähigkeit und Zytotoxizitätsexperimente für Anwendungen in der Krebs-, Neurowissenschaft und Stammzellforschung.

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Global Trade Item Number

SKUGTIN
1164480700104061838259042
501594400104061838259080