PureProteome Magnetic Stand

Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.

PureProteome™ Protein A and G Magnetic beads provide a rapid and reproducible means to purify immunoglobulins (IgG) using the KingFisher Duo particle processor.

Vergleich von Elutionstechniken für die Proteinaufreinigung von FLAG® Tag Proteinen im kleinen Maßstab mittels anti-FLAG® M2 Magnetperlen.

Biomarkers offer important information about homeostasis, disease, response to drug treatments, and environmental stimuli. Sera are rich sources of biomarkers (biological indicator proteins, peptides, small molecules, etc.) and are easier to sample than other tissues. However, the complexity of serum and the presence of highly abundant proteins like albumin and immunoglobulin can mask less abundant species, hindering biomarker detection. PureProteome albumin magnetic beads remove more than 98% of albumin from human serum. Here, we demonstrate that PureProteome albumin magnetic beads may also be used to remove albumin from mouse, guinea pig and rat sera. Depleted samples are often dilute, and may need concentration for downstream analyses. Therefore, we present a protocol for the convenient concentration of these samples using Amicon Ultra 2 mL centrifugal filters.

Purification of recombinant proteins expressed in E.coli requires many time-consuming steps. To liberate the protein of interest, traditional bacterial lysis relies on the addition of lysozyme and a combination of sonication and repeated freeze/thaw cycles to break the bacterial cell wall. Disruption of the cell is accompanied by an increase in the viscosity of the suspension, due to the release of DNA. An endonuclease is added to digest the DNA, thus reducing the viscosity of the lysate. Finally, to render the lysate compatible with traditional purification methods, insoluble cell debris must be removed by centrifugation.