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W1754

Eau

Name: Eau
Brand: SIGMA

PCR Reagent, suitable for PCR

Synonyme(s) :

H₂O

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A propos de cet article

Formule linéaire :

H₂O

Numéro CAS:

7732-18-5

Poids moléculaire :

18.02

NACRES:

NA.25

PubChem Substance ID:

24900779

UNSPSC Code:

12191602

EC Number:

231-791-2

MDL number:

MFCD00011332

Beilstein/REAXYS Number:

2050024

Technique(s):

PCR: suitable

Bp:

100 °C (lit.)

Vapor pressure:

3 mmHg

Service technique

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Propriétés

Nom du produit

Eau, PCR Reagent

vapor density

<1 (vs air)

Quality Level

200

vapor pressure

3 mmHg

sterility

sterile-filtered

form

liquid

packaging

vial of 1.5 mL

technique(s)

PCR: suitable

refractive index

n20/D 1.34 (lit.)

pH

5-7

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

foreign activity

DNase, none detected, RNase, none detected

SMILES string

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

Description

General description

PCR grade water is sterile-filtered. It is free from exonucleases (DNAse, RNAse) and endonuclease (NICKase) and is also free from nucleic acid contamination.

Application

Suitable for polymerase chain reaction (PCR)

Water has been used:

as a component of the reaction mixture and as a diluting agent in microfluidic RT-qPCR
as a component of the reaction mixture for the amplification of products from fungal (Trametes versicolor) DNA
as a diluting agent and as a component of the reaction mixture for the amplification of cDNA
Water has been used to make up the final volume of the sample in polymerase chain reaction (PCR)

Other Notes

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Classe de stockage

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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Protocoles

Long & Accurate PCR with RedAccuTaq®

REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.

Antibody-Enzyme Mediated Hot Start PCR Protocol

JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.

Hot Start dNTP Protocol

Hot start dNTP protocol enhances specificity in PCR by blocking DNA polymerase nucleotide incorporation during PCR.

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Small interfering RNAs (siRNAs) are powerful tools for gene expression knockdown, widely used in molecular biology.

Contaminations occurring in fungal PCR assays.

J Loeffler et al.

Journal of clinical microbiology, 37(4), 1200-1202 (1999-03-13)

Successful in vitro amplification of fungal DNA in clinical specimens has been reported recently. In a collaboration among five European centers, the frequency and risk of contamination due to airborne spore inoculation or carryover contamination in fungal PCR were analyzed.

Identification of Naegleria fowleri in domestic water sources by nested PCR.

Francine Marciano-Cabral et al.

Applied and environmental microbiology, 69(10), 5864-5869 (2003-10-09)

The free-living amoeboflagellate Naegleria fowleri is the causative agent of primary amoebic meningoencephalitis (PAM), a rapidly fatal disease of the central nervous system. In the United States, the disease is generally acquired while swimming and diving in freshwater lakes and

If you?ve seen one worm, have you seen them all? Spatial, community, and genetic variability of tubificid communities in Montana

Lodh N, et al.

Freshwater science, 34(3), 909-917 (2015)

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Numéro d'article de commerce international

Référence	GTIN
W1754-1VL	04061837488689
W1754-5VL	04061837488696