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MilliporeSigma

TP0100

Total Protein Kit, Micro

Synonyme(s) :

Bradford

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A propos de cet article

NACRES:
NA.32
UNSPSC Code:
12352202
Service technique
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description

wavelength 595 nm
total test time 2 min
working reagent stability 18-26°C for 2 weeks
dynamic range 50 mg/dL 0.5 g/L

Quality Level

parameter

18-26 °C temperature, 50 μL sample volume

storage temp.

2-8°C

General description

Protein determination is one of the most common operations performed in biochemical research. Total protein kit, micro provides a simple, rapid, and highly sensitive method requiring only 50 μL or less sample.

Application

Total protein kit, micro has been used to determine:
  • total protein in the bacterial homogenate by Bradford method
  • total protein content by the Bradford microassay from the tissue extracts
  • serum total protein
  • soluble protein content by the Bradford method from leaves

Colorimetric, Endpoint
Suitable for protein determination for samples of up to 0.5 mg/ml.

Biochem/physiol Actions

Brilliant blue G (Coomassie blue) reacts with protein in an acid-alcohol medium to form a blue-colored protein dye complex. The color, measured at 595 nm, is proportional to the protein concentration.
Total protein kit, micro can be used to determine the concentration of proteins in solution.


Composants de kit également disponibles séparément

Réf. du produit
Description
FDS & Tarif

  • Protein Standard 5 mL
    FDS

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1

target_organs

Eyes,Central nervous system

Classe de stockage

3 - Flammable liquids

flash_point_f

132.8 °F

flash_point_c

56 °C

wgk

WGK 3



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Lot/Batch Number

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Joshua M Halman et al.
Tree physiology, 33(11), 1242-1251 (2013-12-05)
Forests of northeastern North America have been exposed to anthropogenic acidic inputs for decades, resulting in altered cation relations and disruptions to associated physiological processes in multiple tree species, including sugar maple (Acer saccharum Marsh.). In the current study, the
Digital camera-based lipase biosensor for the determination of paraoxon
Pohanka M, et al.
Sensors and Actuators B, Chemical, 273, 610-615 (2018)
Differences in antioxidant mechanisms in grapevines subjected to drought and enhanced UV-B radiation.
Doupis G, et al.
Emirates Journal of Food and Agriculture, 24(6) (2012)



Numéro d'article de commerce international

RéférenceGTIN
TP0100-1KT04061837397998