Trypsin inhibitor

Monomer has an apparent molecular weight of approx. 9,000 Da but undergoes a concentration and pH-dependent dimerization.

Trypsin inhibitor from Phaseolus limensis (lima bean) has been used:

• in the inhibition of trypsin in human plasma
• in the termination of proteolytic digestion in rabbit skeletal muscles
• in the inhibition of salivary glands enzyme extract from Lygus lineolaris
• in the inhibition of proteolytic activity in Agave tequilana enzyme extract

Trypsin inhibitor has been used as an affinity ligand for isolation of elastase-type enzymes.

Trypsin inhibitor from lima beans belongs to Bowman-Birk family of protease inhibitors. It is 9 kDa in molecular weight and contains nine disulfide bridges and is highly thermostable. It has trypsin and chymotrypsin binding subdomains.

Prepared by a modification of the method of Tauber, H., et al., J. Biol. Chem., 179, 1155 (1949) (modified).

One mg will inhibit ≥0.8 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.

One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.

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