T3069
Trizma® hydrochloride solution
pH 8.0, BioPerformance Certified, 2 M, suitable for cell culture
Synonyme(s) :
Tris hydrochloride solution
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A propos de cet article
NACRES:
NA.25
UNSPSC Code:
12161700
Service technique
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grade
BioPerformance Certified, Molecular Biology
form
solution
concentration
2 M
technique(s)
cell culture | mammalian: suitable
impurities
DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, ≤5 ppm Heavy metals (as Pb)
color
colorless
pH
7.95—8.05, 8.0
useful pH range
7.0-9.0
density
1.07—1.09 g/cm3
absorption
≤0.05 at 290 at 40%
General description
Tris(hydroxymethyl)aminomethane (Tris) has a pH buffering range between 7.0 - 9.0 and is a routine buffer in biological sciences. Tris is used majorly in combination with ethylenediaminetetraacetic acid (EDTA), especially for electrophoretic studies. It displays negligible binding towards metal in solutions.
A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.
Application
Trizma® hydrochloride solution has been used as a component of lysis buffer for testing on commercially available biological sample preservation paper types. It has also been used as a vehicle and for dissolving leptin in reporter gene assay studies.
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
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(Un) suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions-a review
Ferreira CMH, et al.
Royal Society of Chemistry Advances, 5(39) (5)
Optimal DNA extractions from blood on preservation paper limits conservation genomic but not conservation genetic applications
Stowell SML, et al.
Journal for nature conservation, 46 (46)
Universal buffers for use in biochemistry and biophysical experiments
Brooke D, et al.
AIMS Biophysics, 2(3) (2)
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| T3069-100ML | 04061838175762 |
| T3069-1L | 04061838260215 |