T2663
Trizma® hydrochloride solution
Molecular Biology, 1 M, BioReagent
Synonyme(s) :
Tris hydrochloride solution
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Molecular Biology
Quality Level
sterility
0.2 μm filtered
product line
BioReagent
form
solution
concentration
1 M
impurities
DNase, RNase, NICKase and protease, none detected
pH
7.35-7.45
SMILES string
Cl.NC(CO)(CO)CO
InChI
1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
InChI key
QKNYBSVHEMOAJP-UHFFFAOYSA-N
General description
Trizma® hydrochloride solution is a useful biological buffer.
Application
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Trizma® hydrochloride along with Trizma-base is used to prepare buffer solutions of pH 7.35. It was used to prepare the triton extract. It may be used in the analysis of protease activity, isolated from the Bacillus stearothermophilus and Bacillus subtilis. It may be employed as buffer for the restriction fragment analysis of genomic DNA isolated from the enterococcal isolates and Escherichia coli.
Other Notes
Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Classe de stockage
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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M Fujii et al.
Journal of bacteriology, 154(2), 831-837 (1983-05-01)
The structural gene for a thermostable protease from Bacillus stearothermophilus was cloned in plasmid pTB90. It is expressed in both B. stearothermophilus and Bacillus subtilis. B. stearothermophilus carrying the recombinant plasmid produced about 15-fold more protease (310 U/mg of cell
B E Murray et al.
Journal of clinical microbiology, 28(9), 2059-2063 (1990-09-01)
Epidemiologic evaluation of enterococci has been limited by the lack of a simple and effective method for comparing strains. In this study, we have compared chromosomal restriction endonuclease digestion patterns of 27 isolates of Enterococcus faecalis from three different locations
Reconstitution and purification of the D-glucose transporter from human erythrocytes.
M Kasahara et al.
The Journal of biological chemistry, 252(20), 7384-7390 (1977-10-25)
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| T2663-1L | 04061837342721 |
| T2663-100ML | 04061838238313 |
| T2663-10L | 04061838244949 |