T1819
Trizma® hydrochloride solution
pH 7.0, BioPerformance Certified, 1 M, suitable for cell culture
Synonyme(s) :
Tris hydrochloride solution
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A propos de cet article
NACRES:
NA.25
UNSPSC Code:
12161700
Service technique
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grade
BioPerformance Certified, Molecular Biology
form
liquid
concentration
1 M
technique(s)
cell culture | mammalian: suitable
impurities
DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, ≤5 ppm Heavy metals (as Pb)
pH
7.0
useful pH range
7.0-9.0
absorption
≤0.05 at 290 at 40%
General description
Tris(hydroxymethyl)aminomethane (Tris) is a standard buffer in biological sciences and has a pH buffering range between 7.0 - 9.0. It is applicable in electrophoretic studies and capillary electrochromatography.
A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.
Application
Trizma® hydrochloride solution has been used:
- as a buffer in reverse transcription and DNase I treatment reactions for RNA isolated from Toxoplasma gondii
- in the preparation of tris- ethylenediaminetetraacetic acid (EDTA) (TE) buffer for bacterial pellet resuspension for plasmid isolation studies
- for polylactic acid nanoparticle resuspension, storage, and pH-based stability studies
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Classe de stockage
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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A review of acellular immersion tests on bioactive glasses-influence of medium on ion release and apatite formation
Nommeots-Nomm A, et al.
International Journal of Applied Glass Science, 11(3) (11)
Kaichun Zhu et al.
Nature protocols, 1(6), 3088-3093 (2007-04-05)
This protocol describes a streamlined method of plasmid DNA extraction by continual thermal lysis, a modification of the basic boiling lysis technique, to simplify the processing of large volumes of Escherichia coli cultures. Fermented bacteria are harvested using a hollow
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| T1819-100ML | 04061837338274 |
| T1819-1L | 04061838260185 |