Lyticase from Arthrobacter luteus

Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation.

Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species.

Purified Lyticase SAE0098 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.

La lyticase hydrolyse le poly-β(1→3)-glucose, notamment le glucane de la paroi cellulaire des levures.

Pour en savoir plus sur les enzymes utilisées dans l′analyse des glucides complexes, rendez-vous sur www.sigma-aldrich.com/enzymeexplorer.

Une unité produit une ΔA₈₀₀ de 0,001 par minute à pH 7,5 et 25 °C, en utilisant comme substrat une suspension de levure dans un mélange réactionnel de 3 ml.