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S9811

S-Gal®

reagent for selection of recombinant bacterial clones

Synonyme(s) :

3,4-Cyclohexenoesculetin β-D-galactopyranoside

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A propos de cet article

Formule empirique (notation de Hill) :
C19H22O9
Numéro CAS:
182805-65-8
Poids moléculaire :
394.37
UNSPSC Code:
12352200
PubChem Substance ID:
329824635
NACRES:
NA.85
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grade

Molecular Biology

Quality Level

100

sterility

non-sterile

assay

≥95% (HPLC)

form

powder

solubility

DMF: 50 mg/mL

suitability

suitable for β-galactosidase test

storage temp.

room temp

SMILES string

OC[C@H]1O[C@@H](Oc2cc3OC(=O)C4=C(CCCC4)c3cc2O)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C19H22O9/c20-7-14-15(22)16(23)17(24)19(28-14)27-13-6-12-10(5-11(13)21)8-3-1-2-4-9(8)18(25)26-12/h5-6,14-17,19-24H,1-4,7H2/t14-,15+,16+,17-,19-/m1/s1

InChI key

HDJJDRXZHJRVGA-DIKXUDHVSA-N

General description

S-Gal® is an autoclavable, chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. S-Gal® is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Application

Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. S-Gal® is autoclavable and can be added to bacterial broth containing agar prior to autoclaving.
S-Gal is a patented autoclavable chromogenic substrate for β-galactosidase that is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Biochem/physiol Actions

When S-Gal® is cleaved by β-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal® and ferric ion turn an intense black color, allowing for easy differentiation between lac+ and lac- colonies.

Features and Benefits

  • More intense color contrast than X-gal
  • Excellent for use in automated colony counters
  • Autoclavable for easiest use
  • No need to make stock solutions
When S-Gal is cleaved by β-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal and ferric ion turn an intense black color, allowing for easy differentiation between lac+ and lac- colonies. S-Gal is autoclavable and can be added to your medium of choice prior to autoclaving. S-Gal is not light sensitive and does not require any protection from light sources.

Preparation Note

Stock solutions of S-Gal® can be made by dissolving 50mg/ml in dimethyl formamide (DMF) and storing at -20C. Add S-Gal® (300 mg/L from stock solution ) and Ferric Ammonium Citrate (500mg/L) to agar media prior to autoclaving.

Other Notes

Autoclavable, microwavable growth medium complete with S-Gal and IPTG.
The ferric or Fe3+ ion is required for color development and must be added to any S-Gal®
formulation. A medium prepared with S-Gal® is moderately dark due to the presence of ferric ammonium citrate. This darker background often provides enhanced contrast for automated colony counting or isolation.

Legal Information

S-GAL is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificats d'analyse (COA)

Lot/Batch Number
100K11311
100K11301

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Protocoles

Competent Cell Protocols

Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.

Articles

Bacterial Transformation Protocols

General protocols for growth of competent cells and their transformation (uptake of DNA).

S Fitzpatrick et al.
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 9(2), 206-209 (2008-12-09)
Rhodnius prolixus is the main vector of Chagas disease in Venezuela, where it is found colonising rural housing consisting of unplastered adobe walls with palm and/or metal roofs. Vector control failure in Venezuela may be due to the invasion of
Development and characterization of ten polymorphic microsatellites isolated from the scallop Argopecten purpuratus.
C J Mandiola-Quililongo et al.
Journal of genetics, 91(1), e12-e14 (2012-05-04)
Christopher Voigt
Synthetic Biology: Methods for part/device characterization and chassis engineering (2011)
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Numéro d'article de commerce international

RéférenceGTIN
S9811-1G04061836962166
S9811-5G04061832745244
S9811-100MG04061838355690