P2192
10X PCR Buffer
Optimized for routine PCR with MgCl2 included
Synonyme(s) :
PCR Buffer Solution, PCR Reaction Buffer
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A propos de cet article
NACRES:
NA.52
UNSPSC Code:
41106306
Service technique
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Laissez-nous vous aiderQuality Level
form
liquid
packaging
vial of 1.5 mL
color
colorless
application(s)
agriculture
foreign activity
DNase, RNase, none detected
storage temp.
−20°C
Application
10X PCR Buffer has been used as a component of the PCR mixture:
- for the amplification of viral DNA
- to amplify genomic DNA to study the methylenetetrahydrofolate reductase (MTHFR) gene mutations in attention deficit hyperactivity disorder (ADHD) individuals
- to amplify 16S-23S rRNA gene internal transcribed spacer (ITS) from Bacillus sp.
Features and Benefits
- Standalone buffer
- Compatible with JumpStart™ Taq DNA Polymerase (D9307), Taq DNA Polymerase from Thermus aquaticus (D1806), and REDTaq® Genomic DNA Polymerase (D8312)
Preparation Note
Composition of the 10× buffer: 100 mM Tris-HCl, pH 8.3 at 25°C; 500 mM KCl; 15 mM MgCl2; 0.01% gelatin
Legal Information
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
Classe de stockage
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Protocoles
Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
Contenu apparenté
Maike Stam et al.
Genetics, 162(2), 917-930 (2002-10-26)
Paramutation is an interaction between alleles that leads to a heritable change in the expression of one allele. In B'/B-I plants, B-I (high transcription) always changes to B' (low transcription). The new B' allele retains the low expression state in
G J Breedveld et al.
Neurology, 59(4), 579-584 (2002-08-28)
Benign hereditary chorea (BHC) is an autosomal dominant disorder that can be distinguished from Huntington disease by its early onset, stable or only slightly progressive course, and absence of mental deterioration. The variation in clinical features is such that its
Ákos Boros et al.
PloS one, 6(12), e29145-e29145 (2011-12-24)
A novel positive-sense, single-stranded RNA (+ssRNA) virus (Halastavi árva RNA virus, HalV; JN000306) with di-cistronic genome organization was serendipitously identified in intestinal contents of freshwater carps (Cyprinus carpio) fished by line-fishing from fishpond "Lőrinte halastó" located in Veszprém County, Hungary.
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| P2192-1VL | 04061834362685 |
| P2192-25ML | 04061834362692 |
| P2192-5ML | 04061835507122 |
| P2192-5VL | 04061834362708 |