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MilliporeSigma

O3008

Oleic Acid-Albumin from bovine serum

liquid, sterile-filtered, BioReagent, suitable for cell culture

Synonyme(s) :

Oleic acid-BSA complex

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A propos de cet article

UNSPSC Code:
12352211
NACRES:
NA.75
MDL number:
Biological source:
bovine
Form:
liquid
Technique(s):
cell culture | mammalian: suitable
Impurities:
endotoxin, tested
Concentration:
100 mg/mL BSA in DPBS, 90-120 mg/mL protein (biuret)
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biological source

bovine

Quality Level

sterility

sterile-filtered

product line

BioReagent

form

liquid

composition

Oleic acid, 2 mol/mol albumin

concentration

100 mg/mL BSA in DPBS, 90-120 mg/mL protein (biuret)

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

Application

Oleic Acid-Albumin from bovine serum has been used: as a component of the Dulbecco′s modified Eagle′s medium (DMEM) to study adipogenic differentiation in chicken embryonic fibroblast cell lines, to study its effects on benign prostate epithelial cells as a component of the cell culture medium to quantify triglycerides in human epithelial colorectal adenocarcinoma cells

Biochem/physiol Actions

Fatty Acid-Albumin - complexes are used as alternative sources of lipids in the development of serum-free media. Fatty acids bind to serum proteins in high proportions. Serum proteins may help in the release of beneficial fatty acids and bind those that are inhibitory. Oleic acid bound to bovine serum albumin (BSA) shows a positive effect on the growth of various cells.


Classe de stockage

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves



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Articles

How the unsaturated fatty acid, oleic acid and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.

Supplement cell culture systems with fatty acids for biomanufacturing heterologous proteins like monoclonal antibodies.

Contenu apparenté


Yinfang Wang et al.
Journal of experimental & clinical cancer research : CR, 38(1), 147-147 (2019-04-07)
Ionizing radiation (IR) therapy is the standard first-line treatment for newly diagnosed patients with glioblastoma (GBM), the most common and malignant primary brain tumor. However, the effects of IR are limited due to the aberrant radioresistance of GBM. Transcriptome analysis
Alejandro Morales et al.
Laboratory investigation; a journal of technical methods and pathology, 101(7), 921-934 (2021-04-08)
Lipid droplet (LD) accumulation in cancer results from aberrant metabolic reprograming due to increased lipid uptake, diminished lipolysis and/or de novo lipid synthesis. Initially implicated in storage and lipid trafficking in adipocytes, LDs are more recently recognized to fuel key
Joonbum Lee et al.
Poultry science, 100(5), 101057-101057 (2021-03-21)
Current research of avian adipogenesis has been dependent on primary preadipocytes culture due to the lack of commercially available immortal preadipocyte cell lines in avian species. In addition to primary stromal vascular cells, primary chicken embryonic fibroblasts (CEF) were suggested



Numéro d'article de commerce international

RéférenceGTIN
O3008-5ML04061834227304