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MilliporeSigma

N2770

p-Nitrophenyl phosphate

SIGMAFAST, chromogenic, tablet

Synonyme(s) :

pNPP tablets

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A propos de cet article

UNSPSC Code:
12352204
NACRES:
NA.83
EC Number:
224-246-5
MDL number:
Service technique
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Nom du produit

SIGMAFAST p-Nitrophenyl phosphate Tablets, tablet, To prepare 20 mL

Quality Level

form

tablet

solubility

water: 0.25 tablet/mL, clear, colorless

storage temp.

−20°C

Application

Developed for use as a soluble substrate for the detection of alkaline phosphatase activity in Enzyme Immunoassays (EIA). pNPP is the substrate of choice in high sensitivity alkaline phosphatase-based EIA assays.One pNPP tablet and one Tris buffer tablet are dissolved in deionized water and provide a ready-to-use substrate. Each SIGMAFAST pNPP tablet set yields a solution containing 1.0 mg/ml pNPP, 0.2 M Tris buffer. EIA applications using pNPP may be read in timed assays or stopped with 3N NaOH for delayed readings. SIGMAFAST require only the addition of distilled or deionized water to prepare an active substrate solution. Tablets are individually packaged in foil packets.

Preparation Note

Each tablet set dissolved in 20 mL deionized water yields a ready-to-use buffered solution containing pNPP.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC


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Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Numéro d'article de commerce international

RéférenceGTIN
N2770-50SET04061835557998
N2770-5SET04061834110491