M5310
Huile minérale
light oil, suitable for mouse embryo cell culture, BioXtra
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A propos de cet article
Technique(s):
cell culture | embryo: suitable
Service technique
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Laissez-nous vous aidersterility
sterile-filtered
Quality Level
product line
BioXtra
form
light oil
storage condition
protect from light
technique(s)
cell culture | embryo: suitable
refractive index
n20/D 1.467 (lit.)
density
0.82-0.88 g/mL at 25 °C
format
neat
storage temp.
room temp
InChI key
AEOVEGJBKQQFOP-DDVLFWKVSA-L
General description
Mineral oil or liquid petroleum is used for conservation of various types of cutltures. The advantages of using mineral oil are that the growth rate is decreased and the evaporation of water from the medium is almost completely eliminated. Thus, sub-culturing is reduced leading to increase in the viability of the culture. The first time mineral oil was used was in 1914, and it was used to overlay bacterial culture.
Application
Mineral oil has been commonly used and recommended for overlaying mouse embryo cultures to prevent drying during the procedure.
Mineral oil is used for the following applications:
Mineral oil is used for the following applications:
- Used for cell viability studies in preparing the culture media or media supplements
- Used tp protect array from evaporation by adding mineral oil around the array
- Used to prepare the needle for microinjection (microinjection needle pulling, sharpening, and loading)
- Used in PCR setup
Features and Benefits
Ready-to-use sterile filtered product conveniently packaged in PET bottles.
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Classe de stockage
10 - Combustible liquids
wgk
WGK 1
flash_point_f
No data available
flash_point_c
No data available
ppe
Eyeshields, Gloves
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Articles
Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture
Slobodan Beronja et al.
Methods in molecular biology (Clifton, N.J.), 961, 351-361 (2013-01-18)
We have recently developed a method for RNAi-mediated gene function analysis in skin (Beronja et al., Nat Med 16:821-827, 2010). It employs ultrasound-guided in utero microinjections of lentivirus into the amniotic cavity of embryonic day 9 mice, which result in
Mineral Oil Overlay for Conservation of Plant Tissue Cultures
Caplin SM
American Journal of Botany, 46(5), 324-329 (1959)
Hanyoup Kim et al.
Lab on a chip, 9(9), 1230-1235 (2009-04-17)
We report high-speed real-time PCR performed on an unmodified disposable polystyrene Petri dish. The reaction cycle relies solely on an infrared laser for heating; no conventional heater is required. Nanoliter droplets of PCR mixture as water-in-oil emulsions printed in an
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| M5310-1L | 04061838259721 |
| M5310-100ML | 04061834059806 |
| M5310-500ML | 04061838259738 |