M3024
Milieu essentiel minimum d′Eagle
with Earle′s salts and non-essential amino acids, without L-glutamine, phenol red and sodium bicarbonate, Modified, powder, suitable for cell culture
Synonyme(s) :
MEM
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A propos de cet article
NACRES:
NA.75
UNSPSC Code:
12352207
Service technique
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Laissez-nous vous aiderQuality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
Earle’s salts (5% CO2): yes
L-glutamine: no
HEPES: no
NaHCO3: no
sodium pyruvate: no
phenol red: no
shipped in
ambient
storage temp.
2-8°C
General description
Minimum Essential Medium Eagle contains 19 amino acids. The essential amino acids and the non-essential amino acids: L-ala, L-asn, L-asp, L-glu, L-gly, L-pro, and L-ser. This medium is preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium. It is suitable for a wide variety of cells grown in monolayers.
Application
Minimum Essential Medium Eagle has been used:
- to culture endometrial adenocarcinoma Ishikawa cells for bisphenol studies
- to culture MCF-7 cells for experimental treatments with hormone
- to maintain zebrafish pineal glands for action spectrum analysis
Features and Benefits
Contains high concentrations of amino acids so the medium resembles the protein composition of cultured mammalian cells
Preparation Note
Formulated to contain 9.3 grams of powder per liter of medium.
Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate.
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Classe de stockage
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocoles
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
Articles
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
Contenu apparenté
Kyle S Enger et al.
Journal of water and health, 14(6), 950-960 (2016-12-14)
Many different household water treatment (HWT) methods have been researched and promoted to mitigate the serious burden of diarrheal disease in developing countries. However, HWT methods using bromine have not been extensively evaluated. Two gravity-fed HWT devices (AquaSure™ and Waterbird™)
Anatoli Y Kabakov et al.
PloS one, 10(4), e0123718-e0123718 (2015-04-24)
During long-term potentiation (LTP) of excitatory synapses, Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) is activated by Ca(2+) influx through NMDA receptors that potentiate AMPA receptor currents by insertion of additional GluR1-containing receptors at the synapse and by increasing AMPA channel conductance
Hongjie Fan et al.
Cells, 10(11) (2021-11-28)
Bisphenol A (BPA) is a well-known endocrine disruptor, widely used in various consumer products and ubiquitously found in air, water, food, dust, and sewage leachates. Recently, several countries have restricted the use of BPA and replaced them with bisphenol S
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| M3024-10L | 04061834046301 |
| M3024-10X1L | 04061834046318 |
| M3024-1L | 04061837473449 |