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M1018

Milieu essentiel minimum d’Eagle

Name: Milieu essentiel minimum d’Eagle
Brand: SIGMA

With Hanks′ salts, ʟ-glutamine and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

Synonyme(s) :

MEM

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A propos de cet article

UNSPSC Code:

12352207

NACRES:

NA.75

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Propriétés

Nom du produit

Milieu essentiel minimum d′Eagle, With Hanks′ salts, L-glutamine and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

phenol red: yes
NaHCO₃: no
HEPES: no
sodium pyruvate: no
Hanks’ salts (2% CO₂): yes
L-glutamine: yes

shipped in

ambient

storage temp.

2-8°C

Description

General description

Modification of the original MEM formulation. Earle′s salts have been replaced with Hanks′ salts. Since the bicarbonate concentration is substantially lower, this medium has a substantially reduced buffering capacity. It contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.

Preparation Note

Formulated to contain 10.7 grams of powder per liter of medium.

Supplement with 0.35 g/L sodium bicarbonate.

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Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Protocoles

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Articles

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Contenu apparenté

Minimum Essential Medium Eagle (MEM) Specifications

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Minocycline, an FDA-approved second-generation semisynthetic tetracycline, exerts antioxidant, anti-apoptotic and anti-inflammatory effects, independent of its antimicrobial properties. Interleukin (IL)-17A is an immune and inflammatory mediator, and its sustained induction is associated with various cardiovascular diseases. Here we investigated (i) whether

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Numéro d'article de commerce international

Référence	GTIN
M1018-10L	04061834037712
M1018-1L	04061837473258