M0769
Milieu essentiel minimum d′Eagle
AutoMod™, with Earle′s salts, without L-glutamine and sodium bicarbonate, powder, suitable for cell culture
Synonyme(s) :
MEM
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A propos de cet article
NACRES:
NA.75
UNSPSC Code:
12171500
Service technique
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form
powder
technique(s)
cell culture | mammalian: suitable
components
NaHCO3: no
phenol red: yes
Earle’s salts (5% CO2): yes
L-glutamine: no
sodium pyruvate: no
HEPES: no
shipped in
ambient
storage temp.
2-8°C
General description
Modification of the original MEM formulation. Contains a succinate buffering system. See formulations table for specific information. Medium may be autoclaved.
Preparation Note
Formulated to contain 9.4 grams of powder per liter of medium.
Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate.
Other Notes
Modified for autoclaving.
Legal Information
Auto-Mod is a trademark of Sigma-Aldrich Co. LLC
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Classe de stockage
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocoles
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
Articles
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
Contenu apparenté
Simona Giunta et al.
The Journal of cell biology, 190(2), 197-207 (2010-07-28)
The signaling cascade initiated in response to DNA double-strand breaks (DSBs) has been extensively investigated in interphase cells. Here, we show that mitotic cells treated with DSB-inducing agents activate a "primary" DNA damage response (DDR) comprised of early signaling events
Pasqualino de Antonellis et al.
PloS one, 6(9), e24584-e24584 (2011-09-21)
Through negative regulation of gene expression, microRNAs (miRNAs) can function as oncosuppressors in cancers, and can themselves show altered expression in various tumor types. Here, we have investigated medulloblastoma tumors (MBs), which arise from an early impairment of developmental processes
Carlos Mendoza-Palomares et al.
Eukaryotic cell, 7(4), 684-697 (2008-02-19)
Cysteine proteases have been shown to be essential virulence factors and drug targets in trypanosomatids and an attractive antidisease vaccine candidate for Trypanosoma congolense. Here, we describe an important amplification of genes encoding cathepsin B-like proteases unique to T. congolense.
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| M0769-50L | 04061837459566 |
| M0769-10X1L | 04061834037415 |
| M0769-1L | 04061837459559 |