Accéder au contenu
MilliporeSigma

Principaux documents

Voir toute la documentation

M0275

Milieu essentiel minimum d’Eagle

With Earle′s salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10x

Synonyme(s) :

MEM

Se connecter pour consulter les tarifs organisationnels et contractuels.

Sélectionner une taille de conditionnement

Changer de vue

A propos de cet article

UNSPSC Code:
12352207
NACRES:
NA.71
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aider

Nom du produit

Milieu essentiel minimum d′Eagle, 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: yes
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no
sodium pyruvate: no
NaHCO3: no

shipped in

ambient

storage temp.

2-8°C

Application

Minimum Essential Medium Eagle has been used for the formulation of collagen methacrylamide (hydrogel). It has also been used to maintain articular cartilage of bovine knee joints, which was later used for MRI (magnetic resonance imaging) study and histological analysis.
Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera. The L-glutamine and sodium bicarbonate are added to the medium after dilution to 1x and pH adjustment.

Preparation Note

Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate at 1×.

Still not finding the right product?

Explore all of our products under Milieu essentiel minimum d′Eagle

Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
088K2312
048K2438
117K2300
097K2323
066K2409

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Articles

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Collagen-lactoferrin fibrillar coatings enhance osteoblast proliferation and differentiation
Vandrovcova M, et al.
Journal of Biomedical Materials Research Part A, 103(2), 525-533 (2015)
Comparison of morphology, orientation, and migration of tendon derived fibroblasts and bone marrow stromal cells on electrochemically aligned collagen constructs
Gurkan UA, et al.
Journal of Biomedical Materials Research Part A, 94(4), 1070-1079 (2010)
Karl Norris et al.
Micromachines, 10(1) (2019-01-24)
Polysaccharides of marine origin are gaining interest as biomaterial components. Bacteria derived from deep-sea hydrothermal vents can produce sulfated exopolysaccharides (EPS), which can influence cell behavior. The use of such polysaccharides as components of organic, collagen fibril-based coatings on biomaterial
Afficher tous les articles scientifiques apparentés

Numéro d'article de commerce international

RéférenceGTIN
M0275-6X500ML04061833992951
M0275-100ML04061833992937
M0275-500ML04061837847257