M0268
Milieu essentiel minimum d′Eagle
With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Synonyme(s) :
MEM
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A propos de cet article
NACRES:
NA.75
UNSPSC Code:
12352207
Service technique
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powder
technique(s)
cell culture | mammalian: suitable
components
HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
L-glutamine: yes
sodium pyruvate: no
shipped in
ambient
storage temp.
2-8°C
General description
Minimum Essential Medium Eagle (MEM) is one of the most widely used synthetic cell culture media. MEM has been used for culturing several cells grown in monolayers. It is commonly used to grow attached cell lines, such as fibroblasts, in the presence of fetal bovine sera (FBS), calf, or horse sera.
Application
Minimum Essential Medium Eagle has been used:
- in the isolation and culture of primary hepatocytes
- to culture the human endometrial carcinoma cells Ishikawa (ATTC) and L-Wnt-3a cells (ATCC) that secrete Wnt family member 3a (Wnt-3a) ligand
- to culture the AN3CA (endometrial cancer cell line) cells, and HEC1-B human (endometrial adenocarcinoma cell line) cells
Features and Benefits
- Minimum Essential Medium Eagle (MEM) contains higher concentrations of amino acids to mimic the protein composition of cultured mammalian cells.
- The formulation has been altered by optional elimination of calcium to enable the growth of cells in suspension culture.
Preparation Note
Formulated to contain 9.5 grams of powder per liter of medium.
Supplement with 2.2 g/L sodium bicarbonate.
Other Notes
Essentially the same as the original formula, except it is provided as a powder.
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Classe de stockage
13 - Non Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocoles
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
Articles
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
Contenu apparenté
T L Freeman et al.
Biochemical pharmacology, 68(2), 223-230 (2004-06-15)
Activation of hepatic stellate cells (HSCs) involves the induction of ECM protein synthesis and rapid cell proliferation. Thus, agents that interfere with either process could potentially mitigate the development of liver disease by reducing the synthesis of proteins associated with
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The poor mechanical properties and processability of pristine heterocyclic conductive polymers represent the most notable scientific and technological challenges that have greatly limited the application of these polymers. We report a soft and mechanically processable free-standing pristine polypyrrole (PPy) membrane
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As one of the most frequent diabetic complications, diabetic foot ulcer (DFU) can cause limb ischemia or even amputation. Paeoniflorin (PF) has been reported to possess many kinds of biological functions, such as antioxidant and anti-inflammatory effects. However, the role
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| M0268-100L | 04061837473111 |
| M0268-10L | 04061835568772 |
| M0268-10X1L | 04061835568789 |
| M0268-1L | 04061837473128 |
| M0268-50L | 04061833992906 |