I7633
IMDM
With ʟ-glutamine and HEPES, without sodium bicarbonate, powder, suitable for cell culture
Synonyme(s) :
IMDM
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Milieu de Dulbecco modifié par Iscove, With L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
form
powder
technique(s)
cell culture | hybridoma: suitable, cell culture | mammalian: suitable
components
phenol red: yes
glucose: yes
L-glutamine: yes
shipped in
ambient
storage temp.
2-8°C
General description
Iscove′s Modified Dulbecco′s medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids, and vitamins, sodium pyruvate, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, and potassium nitrate instead of ferric nitrate. Iscove′s medium supports murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.
Application
Iscove′s Modified Dulbecco′s Medium has been used to culture KG1a cells (human leukemic cell line) and Chinese hamster ovary (CHO) DG44 cell line.
Reported to support murine B lymphocytes, hematopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.
Preparation Note
Formulated to contain 17.7 grams of powder per liter of medium.
Supplement with 3.024 g/L sodium bicarbonate.
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Classe de stockage
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocoles
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
Contenu apparenté
Lucía Flores-Peredo et al.
Molecular carcinogenesis, 56(2), 735-750 (2016-07-20)
The Sarco/Endoplasmic Reticulum Ca
Hale M Tasyurek et al.
Human gene therapy, 29(7), 802-815 (2018-02-08)
Postprandial glucose-induced insulin secretion from the islets of Langerhans is facilitated by glucagon-like peptide-1 (GLP-1)-a metabolic hormone with insulinotropic properties. Among the variety of effects it mediates, GLP-1 induces delta cell secretion of somatostatin, inhibits alpha cell release of glucagon
Ryan S Pawell et al.
Biomicrofluidics, 7(5), 56501-56501 (2014-01-10)
Deterministic lateral displacement (DLD) is a microfluidic size-based particle separation or filter technology with applications in cell separation and enrichment. Currently, there are no cost-effective manufacturing methods for this promising microfluidic technology. In this fabrication paper, however, we develop a
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| I7633-10X1L | 04061833858332 |
| I7633-10L | 04061833858325 |
| I7633-50L | 04061833858349 |