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H2126

Hyaluronidase from sheep testes

Type II, lyophilized powder, ≥300 units/mg solid

Synonyme(s) :

Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase

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A propos de cet article

Numéro CAS:
37326-33-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-464-3
MDL number:
MFCD00131351
Numéro CE :
3.2.1.35 (BRENDA, IUBMB)
Specific activity:
≥300 units/mg solid
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conjugate

conjugate (Glucosaminoglycan)

Quality Level

200

type

Type II

form

lyophilized powder

specific activity

≥300 units/mg solid

mol wt

55 kDa

storage temp.

−20°C

General description

Hyaluronidase is a hyaluronic acid-metabolizing enzyme. It is found in the venoms of snake, honeybee and hymenoptera. Hyaluronidase degrades hyaluronic acid (HA), which enhances membrane permeability, lowers viscosity and increases tissue permeability. It enhances the diffusion of local anesthetics, lowers the size of myocardial infarction, improves the effects of anticancer drugs and prevents perineal trauma during spontaneous delivery.

Application

Hyaluronidase from sheep testes has been used:
  • in flow cytometry
  • in the isolation of putative spermatogonial stem cells (SSCs)
  • to study the effects of magnetic stimulation in magnetic hydrogel properties
  • in immunohistochemistry to visualize collagen type II distribution
  • to expose group-III auditory neurons of Muller′s organ for patch-clamp recordings
  • for the degeneration of hydrogels

Biochem/physiol Actions

These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Physical form

Lyophilized powder containing lactose

Other Notes

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
098K7016
058K7353
037K7020
126K7008
046K7014

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Protocoles

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

Articles

What is Hyaluronan?

Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.

Glycosaminoglycans and Proteoglycans

Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

Isolation and enrichment of putative spermatogonial stem cells from ram (Ovis aries) testis
Binsila KB, et al.
Animal Reproduction Science, 609-620 (2018)
Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture
Otto IA, et al.
European Cells & Materials, 35, 132-150 (2018)
Miscellaneous Drugs, Materials, Medical Devices and Techniques
Nan A
Side Effects of Drugs Annual, 37, 603-619 (2015)
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Numéro d'article de commerce international

RéférenceGTIN
H2126-5G04061833791820
H2126-100MG04061833791790
H2126-1G04061833791806
H2126-500MG04061833791813