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GE17-0041-01

Sephadex® G-50

Superfine, Cytiva, pack of 100 g

Synonyme(s) :

SEC resin, buffer exchange resin, chromatography resin, cross-linked dextran, cytiva 17-0041-01, desalting matrix, filtration gel, size-exclusion resin

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A propos de cet article

Numéro CAS:
9004-54-0
UNSPSC Code:
41115722
NACRES:
NA.56
MDL number:
MFCD23701222.obs1210407
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form

resin

crosslinking

cross-linked

mol wt

protein Mr 3-20 kDa (fractionation range), protein Mr 30 kDa (exclusion limit)

feature

autoclavable In wet form (pH 7.0) at 120°C for 30 min

packaging

pack of 100 g

manufacturer/tradename

Cytiva

technique(s)

liquid chromatography (LC): suitable

matrix

cross-linked dextran

matrix active group

dextran

swelling

1 g swells to 12 mL

particle size

25-50 μm (dry), 51-106 μm (wet)

cleaning in place

2-13

working range

2-12

separation technique

size exclusion (SEC)

shipped in

ambient

storage temp.

2-30°C

SMILES string

O1C(C(C(C(C1CO)O)O)O)OCC2OC(C(C(C2O)O)O)OCC(O)C(O)C(O)C(O)C=O

InChI

1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2

InChI key

FZWBNHMXJMCXLU-UHFFFAOYSA-N

General description

Sephadex® G-50 Superfine is a well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. The Superfine´s small bead size give higher efficiency.

Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.

Features and Benefits

  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  • Suitable for purification of DNA from small molecules by gel filtration
  • Small bead size for shorter diffusion distances
  • Smallest bead size in the Sephadex® G-50 range

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sephadex is a registered trademark of Cytiva

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Classe de stockage

13 - Non Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Contenu apparenté

Sephadex® and Darcy’s Law

Sephadex® from Cytiva and Darcy’s law in Size Exclusion Chromatography

Meriem Ameziani et al.
The protein journal, 39(5), 574-590 (2020-09-23)
The current report focuses on purification, structural and functional characterization of Cerastategrin from Cerastes cerastes venom, a novel basic disintegrin (pI 8.36) with 128 amino acid residues and a molecular weight of 13 835.25 Da measured by MALDI-MSMS. The 3D structure

Numéro d'article de commerce international

RéférenceGTIN
GE17-0041-0104061834083382