G5893
Gamborg′s B-5 Basal Medium with Minimal Organics
fine powder, suitable for plant cell culture
Synonyme(s) :
B-5 Basal Medium, Basal Medium, Minimal Organics Medium
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NACRES:
NA.72
UNSPSC Code:
12352207
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fine powder
Quality Level
technique(s)
cell culture | plant: suitable
application(s)
agriculture
storage temp.
2-8°C
General description
Gamborg′s B-5 basal medium is an efficient nutrient mixture for plants.
Gamborg′ B-5 medium may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro culture of plant cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis. Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.
Application
Gamborg′s B-5 Basal Medium with Minimal Organics has also been used:
- as a component of liquid media for the tissue culture of Brassica rapa
- as a component of callus inducing medium for culturing Arabidopsis cardiolipin mutant seedlings
- as a component of solid GM medium for germination of Arabidopsis seeds
Preparation Note
Formulated to contain 3.2 grams of powder per liter of medium.
With the macro- and micronutrients, and vitamins as described by Gamborg, et al. (1968).
Media Formulation
Media Formulation
signalword
Warning
hcodes
Hazard Classifications
Ox. Sol. 3
Classe de stockage
5.1B - Oxidizing hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.
Contenu apparenté
Experiments in Plant Tissue Culture (1985)
Leigh Mickelson-Young et al.
Journal of experimental botany, 67(21), 6077-6087 (2016-11-05)
The duration of the DNA synthesis stage (S phase) of the cell cycle is fundamental in our understanding of cell cycle kinetics, cell proliferation, and DNA replication timing programs. Most S-phase duration estimates that exist for plants are based on
Metabolism of polyamines in transgenic cells of carrot expressing a mouse ornithine decarboxylase cDNA
Andersen S E, et al.
Plant Physiology, 116(1), 299-307 (1998)
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| G5893-10L | 04061833638668 |
| G5893-1L | 04061833638675 |