G2580
Sephadex® G-25
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technique(s)
buffer exchange: suitable
matrix active group
phase
swelling
1 g swells to 4-6 mL
bead size
20-80 μm
application(s)
life science and biopharma
compatibility
Cytiva
SMILES string
O1[C@H]([C@@H]([C@@H]([C@@H]([C@H]1CO)O)O)O)O.OC(CO)CO
InChI
1S/C6H12O6.C3H8O3/c7-1-2-3(8)4(9)5(10)6(11)12-2;4-1-3(6)2-5/h2-11H,1H2;3-6H,1-2H2/t2-,3-,4-,5-,6-;/m1./s1
InChI key
BCPVZFFJGCURNR-OCOFDJSDSA-N
General description
Sephadex® is a beaded gel filtration medium prepared by cross-linking dextran with epichlorohydrin under alkaline conditions. It is used in affinity chromatography, protein chromatography and gel filtration chromatography.
Fractionation Range (MW)
Globular Proteins: 1,000 - 5,000
Dextrans: 100 - 5,000
Fractionation Range (MW)
Globular Proteins: 1,000 - 5,000
Dextrans: 100 - 5,000
Application
Sephadex® G-25 has been used in size-exclusion chromatography to remove excess dye from labelled proteins.
Other Notes
G2580-100G′s updated product number is GE17-0032-01
G2580-500G′s updated product number is GE17-0032-02
G2580-500G′s updated product number is GE17-0032-02
Legal Information
Sephadex is a registered trademark of Cytiva
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Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Celine Hoffmann et al.
Analytical biochemistry, 372(1), 62-71 (2007-09-11)
Fluorescein isothiocyanate (FITC) is a well-known probe for labeling biologically relevant proteins. However, the impact of the labeling procedure on protein structure and biological activities remains unclear. In this work, FITC-labeled human plasma fibronectin (Fn) was developed to gain insight
Jennifer Belyea et al.
Biochemistry, 45(48), 14275-14284 (2006-11-30)
The study of axial ligation by anionic ligands to ferric heme iron by resonance Raman spectroscopy provides a basis for comparison of the intrinsic electron donor ability of the proximal histidine in horse heart myoglobin (HHMb), dehaloperoxidase (DHP), and horseradish
Conformations of a metastable SH3 domain characterized by smFRET and an excluded-volume polymer model.
Mazouchi A, et al.
Biophysical Journal, 110(7), 1510-1522 (2016)
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| G2580-500G | 04061833629048 |
| G2580-100G | 04061833629031 |
| G2580-10G | 04061838551559 |
| G2580-50G | 04061833629079 |