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G2174

β-glucuronidase from limpets (Patella vulgata)

aqueous solution, ≥85,000 units/mL

Synonyme(s) :

β-D-glucuronide glucuronosohydrolase

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A propos de cet article

Numéro CAS:
9001-45-0
UNSPSC Code:
12352204
NACRES:
NA.54
Numéro CE :
3.2.1.31 (BRENDA, IUBMB)
MDL number:
MFCD00130629
Specific activity:
≥85,000 units/mL
Biological source:
limpet (Patella vulgata)
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biological source

limpet (Patella vulgata)

Quality Level

200

form

aqueous solution

specific activity

≥85,000 units/mL

secondary activity

≤7,500 units/mL sulfatase

storage temp.

2-8°C

Application

Nouvel article technique comparant les propriétés de différentes enzymes
Cliquez ici pour découvrir les dernières données techniques produites par la R&D de Sigma afin d'optimiser l'hydrolyse de différentes classes de médicaments à l'aide d'enzymes provenant de différentes sources et l'utilisation d'une enzyme purifiée par chromatographie pour réduire l'activité estérase qui convertit la 6-MAM en morphine.
Il a été observé que l'enzyme de Patella vulgata est plus efficace que les enzymes d'Helix pomatia, de foie de bovin et d'E. coli pour hydrolyser les opioïde-glucuronides.

Features and Benefits

Save time with no reconstitution in this convenient liquid format.

Physical form

Aqueous solution in 0.9% NaCl with 0.02% sodium azide as preservative.

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Classe de stockage

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Protocoles

UHPLC/MS for Drug Detection in Urine

Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.

LC/MS (TOF) Analysis of Drugs and Their Glucuronide Metabolites in Urine on Titan™ C18 After Solid Phase Extraction (SPE) Using Supel™-Select SCX, β-Glucuronidase Enzyme Digestion

-THC solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material

Articles

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Contenu apparenté

J Combie et al.
Clinical chemistry, 28(1), 83-86 (1982-01-01)
beta-Glucuronidase from Patella vulgata, Helix aspersa, Helix pomatia, and bovine liver were evaluated for usefulness in routine hydrolysis of drug-glucuronic acid conjugates from equine urine samples. Factors affecting the reaction rate (enzyme concentration, ligand concentration, temperature, and pH) were optimized.
T A Jennison et al.
Journal of analytical toxicology, 17(4), 208-210 (1993-07-01)
Methods to confirm morphine in urine require hydrolysis to liberate morphine from its 3-beta-D glucuronide (M-3G) conjugate. Lengthy enzyme hydrolysis procedures prolong testing turnaround time whereas rapid enzyme methods may produce a low conversion of M-3G to morphine. The purpose
Victor Aguilar-Hernández et al.
Plant molecular biology, 84(4-5), 429-441 (2013-10-19)
Gene duplication events exert key functions on gene innovations during the evolution of the eukaryotic genomes. A large portion of the total gene content in plants arose from tandem duplications events, which often result in paralog genes with high sequence
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Numéro d'article de commerce international

RéférenceGTIN
G2174-1ML04061838087447
G2174-10ML04061838087430
G2174-25ML04061833628737
G2174-5ML04061838087454