G0376
β-glucuronidase from bovine liver
Type B-3, ≥2,000,000 units/g solid
Synonyme(s) :
β-D-glucuronide glucuronosohydrolase
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A propos de cet article
Numéro CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-606-8
MDL number:
Specific activity:
≥2,000,000 units/g solid
Biological source:
bovine liver
Service technique
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Laissez-nous vous aiderbiological source
bovine liver
Quality Level
type
Type B-3
form
solid
specific activity
≥2,000,000 units/g solid
storage temp.
−20°C
Application
βLa β-glucuronidase est utilisée comme gène rapporteur dans les essais GUS pour évaluer l′expression génique.
Biochem/physiol Actions
βLa β-glucuronidase catalyse la dégradation des glucides complexes. Chez l′homme, elle convertit la bilirubine conjuguée en sa forme non conjuguée, la rendant ainsi réabsorbable.
Other Notes
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
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signalword
Danger
Classe de stockage
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
wgk
WGK 1
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Protocoles
Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.
Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.
Xiaoyan Liu et al.
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences, 42(1), 67-74 (2013-03-19)
To construct a lentiviral RNA interference system targeting heparanase (HPSE) based on miR30 and to test its silencing effect. Three heparanase-shRNA structures were designed based miR30. The targeting fragments were obtained by PCR, then inserted into the vector LV PP-GFP
Klotho to treat kidney fibrosis.
Maria D Sanchez-Niño et al.
Journal of the American Society of Nephrology : JASN, 24(5), 687-689 (2013-04-20)
G Cassinelli et al.
Biochemical pharmacology, 85(10), 1424-1432 (2013-03-08)
The activity of heparanase is responsible for heparan sulfate cleavage, thus resulting in the release of heparan sulfate-bound growth factors. Since heparanase activity is upregulated in several tumor types and is implicated in the malignant behavior, the enzyme is regarded
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| G0376-1MU | 04061833623565 |
| G0376-500KU | 04061833623572 |
| G0376-5MU | 04061832843568 |