FG1215
RPMI-1640 Medium
With stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Synonyme(s) :
Roswell Park Memorial Institute 1640 medium
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UNSPSC Code:
12352207
NACRES:
NA.71
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RPMI-1640 Medium, With L-alanyl-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered, sterile
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
sodium pyruvate: no
NaHCO3: yes
HEPES: no
phenol red: yes
stable glutamine: yes
shipped in
ambient
storage temp.
2-8°C
General description
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.
Application
RPMI-1640 Medium has been used to culture murine fibroblast cells. It has also been used to culture human ovarian and rat substantia nigra cells.
Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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M Granados et al.
Journal of cardiovascular translational research, 10(4), 374-390 (2017-05-04)
Decellularized scaffolds represent a promising alternative for mitral valve (MV) replacement. This work developed and characterized a protocol for the decellularization of whole MVs. Porcine MVs were decellularized with 0.5% (w/v) SDS and 0.5% (w/v) SD and sterilized with 0.1%