FG0465
IMDM
With stable-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Synonyme(s) :
Cell culture medium, Iscove′s MDM, Iscove’s Modified Dulbecco’s Medium, Modified Dulbecco′s medium
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A propos de cet article
UNSPSC Code:
12352207
NACRES:
NA.71
Service technique
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Iscove′s Modified Dulbecco′s Medium, With L-alanyl-L-glutamine and sodium bicarbonate, sterile-filtered, liquid, suitable for cell culture
Quality Level
sterility
sterile-filtered, sterile
product line
AQmedia™
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
Endotoxin, tested
components
stable glutamine: yes
NaHCO3: yes
glucose: yes
phenol red: yes
shipped in
ambient
storage temp.
2-8°C
General description
This IMDM differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.
Application
Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco¢s Modified Eagle¢s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove¢s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.
Legal Information
AQmedia is a trademark of Sigma-Aldrich Co. LLC
Classe de stockage
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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