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F8435

PNGase F Glycosidase

Removes N-linked glycans, suitable for mass spectrometry, recombinant, expressed in E. coli

Synonyme(s) :

N-Glycosidase F, PNGase F, Peptide N-glycosidase

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A propos de cet article

Numéro CAS:
83534-39-8
UNSPSC Code:
12352204
NACRES:
NA.54
Numéro CE :
3.5.1.52 (BRENDA, IUBMB)
MDL number:
MFCD18839214
Specific activity:
≥1,000 U/mg
Recombinant:
expressed in E. coli
Shelf life:
≥1 weeks at 2‑8 °C (for reconstituted solution), ≥1 yr at -20 °C
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Nom du produit

PNGase F from Elizabethkingia meningoseptica, lyophilized powder, recombinant, expressed in E. coli

recombinant

expressed in E. coli

Quality Level

200

conjugate

(N-linked)

grade

Proteomics Grade

form

lyophilized powder

specific activity

≥1,000 U/mg

shelf life

≥1 weeks at 2‑8 °C (for reconstituted solution), ≥1 yr at -20 °C

mol wt

~36 kDa

shipped in

wet ice

storage temp.

−20°C

Application

Produit utilisé pour la déglycosylation des protéines.
Highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-teminal 6x histidine fusion tag.

Biochem/physiol Actions

Clive un glycane entier d′une glycoprotéine, à condition que le fragment d′asparagine glycosylé soit substitué sur ses extrémités amino et carboxyle avec une chaîne polypeptidique.

Packaging

PNGase F was used for deglycosylation of P-glycoprotein in a study to investigate the dual impact of statins on p-glycoprotein and its effect on doxorubicin cytotoxicity in human neuroblastoma cells. It was used to treat a purified protein, mouse cone ultraviolet (MUV) pigment, before use in quantitative immunoblot analysis in a study It is used to deglycosylate N-linked glycoproteins. Highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-teminal 6x histidine fusion tag.

Other Notes

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Protocoles

Released N-Linked Glycan Analysis Workflow of Adalimumab

A step-by-step protocol for released N-linked glycan analysis of the monoclonal antibody adalimumab, based on UHPLC-FLR-MS and procainamide labeling.

Articles

Improving Glycoform Coverage for Mucin Preparations

Pretreatment with Mucinase StcE increases glycopeptide identification from mucin samples, enhancing sample preparation efficiency for glycopeptide analysis.

Strategies for Deglycosylating N-Linked Glycans

Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.

Maria Nordgren et al.
Methods in molecular biology (Clifton, N.J.), 2271, 155-167 (2021-04-29)
O-glycosylation is a difficult posttranslational modification to analyze. O-glycans are labile and often cluster making their analysis by LC-MS very challenging. OpeRATOR is an O-glycan specific protease that cleaves the protein backbone N-terminally of glycosylated serine and threonine residues. This
Lauren L Daniele et al.
Investigative ophthalmology & visual science, 46(6), 2156-2167 (2005-05-26)
To test the hypothesis that Nrl(-)(/)(-) photoreceptors are cones, by comparing them with WT rods and cones using morphological, molecular, histochemical, and electrophysiological criteria. The photoreceptor layer of fixed retinal tissue of 4- to 6-week-old mice was examined in plastic
T H Plummer et al.
The Journal of biological chemistry, 259(17), 10700-10704 (1984-09-10)
Endo-beta-N-acetylglucosaminidase F preparations from Flavobacterium meningosepticum have been found to contain peptide:N-glycosidase activity. Only the second activity, designated as peptide:N-glycosidase F, readily cleaves the beta-aspartylglycosylamine linkage of a fetuin triantennary complex glycopeptide, as shown by the isolation of the corresponding
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Numéro d'article de commerce international

RéférenceGTIN
F8435-300UN04061832755656
F8435-50UN04061833620656