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CS0760

Isolated Mitochondria Staining Kit

1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)

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A propos de cet article

UNSPSC Code:
12352207
EC Number:
217-896-6
NACRES:
NA.32
Technique(s):
flow cytometry: suitable, protein staining: suitable
Usage:
 kit sufficient for 1000 reactions (using 96 multiwell plates),  kit sufficient for 50 reactions (in a 2 mL cuvette)
Storage temp.:
−20°C
Detection method:
fluorometric
Shipped in:
dry ice
Service technique
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Quality Level

200

usage

 kit sufficient for 1000 reactions (using 96 multiwell plates),  kit sufficient for 50 reactions (in a 2 mL cuvette)

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

flow cytometry: suitable, protein staining: suitable

fluorescence

λex 490 nm; λem 590 nm (JC-1 dye)

application(s)

cell analysis
detection

detection method

fluorometric

shipped in

dry ice

storage temp.

−20°C

Application

Isolated Mitochondria Staining Kit has been used to assess mitochondrial inner membrane potential.

Biochem/physiol Actions

The kit is based on mitochondria staining using the JC-1 dye. In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) does not allow the accumulation of JC-1 dye in the mitochondria. The dye is detected using a fluorimeter or fluorescence microscope.

Features and Benefits

  • Fast, simple, and convenient method for staining isolated mitochondria and for assaying mitochondria intactness
  • Contains all the reagents required for the detection of changes in mitochondrial inner-membrane electrochemical potential
  • Contains the antibiotic Valinomycin that permeabilizes the mitochondrial membrane, and thus, dissipates the mitochondrial electrochemical potential. Valinomycin can be used as a control agent that prevents JC-1 aggregation

Analysis Note

The kit measures mitochondria intactness and detects events that dissipate the mitochondrial membrane potential.

Composants de kit seuls

Réf. du produit
Description
  • DMSO 1 mL
  • JC-1 Assay Buffer 5X 25 mL
  • JC-1 Stain 25 μg
  • Valinomycin Ready Made 100 μL

pictograms

Skull and crossbones
GHS06

signalword

Danger

Hazard Classifications

Acute Tox. 1 Dermal - Acute Tox. 1 Oral

Classe de stockage

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

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Certificats d'analyse (COA)

Lot/Batch Number
058K4153
035K4139

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Articles

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Mitochondrial Stress and ROS

Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

Differential expression of choline kinase isoforms in skeletal muscle explains the phenotypic variability in the rostrocaudal muscular dystrophy mouse
Wu G, et al.
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1801(4), 446-454 (2010)
Neuroprotective efficacy of mitochondrial antioxidant MitoQ in suppressing peroxynitrite-mediated mitochondrial dysfunction inflicted by lead toxicity in the rat brain
Maiti AK, et al.
Neurotoxicity Research, 31(3), 358-372 (2017)
Subcellular fractionation of mitochondria.
Rice, J.E., and Lindsay, J.G. et al.
Subcellular Fractionation, A Practical Approach, 107-142 (1997)
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Numéro d'article de commerce international

RéférenceGTIN
CS0760-1KT04061833553138