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MITOISO2

Mitochondria Isolation Kit

sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells

Synonyme(s) :

Mitochondrial Extraction Kit, Mitochondrial Purification Kit

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NACRES:
NA.32
UNSPSC Code:
12352200
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Quality Level

200

usage

sufficient for 50 applications (2-5 x 107 cells)

technique(s)

ELISA: suitable, western blot: suitable

shipped in

dry ice

storage temp.

−20°C

General description

Mitochondria, a double membrane structure, is the site of most energy production in eukaryotic cells. The mitochondria isolation kit contains two buffers: one for extracting proteins for functional studies and another for profiling in proteome studies. Additionally, the kit includes a storage buffer for preserving intact mitochondria.

Application

Mitochondria Isolation Kit has been used in the isolation of mitochondria from:
  • Ewing′s Sarcoma Family of Tumor (ESFT) cells
  • blood samples of tuberculosis patients
  • human neuroblastoma cells and glioma cells
  • myotubes
  • HepG2 cells

Features and Benefits

  • The kit allows for quick and simple extraction of a concentrated portion of mitochondria from cells.
  • The isolated mitochondria will mostly have intact inner and outer membranes.
  • Can be used to extract mitochondrial proteins for proteome analysis

Other Notes

  • This product is for R&D use only, not for drug, household, or other uses.
  • Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
  • Use ultrapure water for the preparation of reagents.
  • Please refer to the product information sheet to learn more about kit components, reagents required, storage and stability, and isolation protocols.

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Composants de kit également disponibles séparément

Réf. du produit
Description
FDS & Tarif
  • C2978
    CelLytic™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization. 10 mL
    FDS
  • P8340
    Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 2.5 mL
    FDS
  • C0356
    Protein Extraction Reagent Type 4 1 bottle
    FDS

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Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2

Classe de stockage

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

wgk

WGK 3

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
068K4126
097K4103
096K4026
085K4052

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Articles

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Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Mitochondrial Stress and ROS

Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Theodora Panagaki et al.
Biomolecules, 10(4) (2020-04-29)
Down syndrome (trisomy of human chromosome 21) is a common genetic disorder. Overproduction of the gaseous mediator hydrogen sulfide (H2S) has been implicated in the pathogenesis of neurological and metabolic deficits associated with Down syndrome. Several lines of data indicate
Shili Duan et al.
The Journal of biological chemistry, 278(2), 1346-1353 (2002-10-31)
We have shown here that the apoptosis inducer staurosporine causes an early decrease in the endogenous respiration rate in intact 143B.TK(-) cells. On the other hand, the activity of cytochrome c oxidase is unchanged for the first 8 h after
The carbonic anhydrase inhibitor methazolamide prevents amyloid beta-induced mitochondrial dysfunction and caspase activation protecting neuronal and glial cells in vitro and in the mouse brain
Fossati S, et al.
Neurobiology of Disease, 86, 29-40 (2016)
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Numéro d'article de commerce international

RéférenceGTIN
MITOISO2-1KT04061834066781