CMC0024
CONTROLLER BL21(DE3) Chemically Competent Cells
Escherichia coli, rod shaped
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A propos de cet article
UNSPSC Code:
12352200
NACRES:
NA.85
Biological source:
Escherichia coli
Growth mode:
adherent or suspension
Morphology:
rod shaped
Service technique
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CONTROLLER BL21(DE3) Chemically Competent Cells, for controlled protein expression
biological source
Escherichia coli
grade
Molecular Biology
growth mode
adherent or suspension
morphology
rod shaped
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: chemically competent
transformation efficiency: >1 x 107 cfu/μg
shipped in
dry ice
storage temp.
−70°C
General description
The CONTROLLER BL21(DE3) Chemically Competent Cells are an E. coli strain optimized for high efficiency transformation.
They are ideal for cloning and propagation of plasmid clones. They give high yield and high quality plasmid DNA.
Genotype
F- ompT hsdSB (rB- mB-) gal dcm (DE3) Mini-F lacIq1(GentR)
They are ideal for cloning and propagation of plasmid clones. They give high yield and high quality plasmid DNA.
Genotype
F- ompT hsdSB (rB- mB-) gal dcm (DE3) Mini-F lacIq1(GentR)
Features and Benefits
- Reduce leaky expression: The LacI repressor protein binds to the lacO operator to block transcription of downstream genes. In the absence of an induction agent such as lactose sugar or IPTG, target protein expression from the adjacent promoter will be greatly reduced.
- High protein yields: Both 10G and BL21(DE3)-based strains are noted for their ability to express high levels of protein. For expression from a T7 promoter please select the HI-Control BL21(DE3) strain. This strain also is deficient in proteases, yielding more stable protein preparations. Most other promoters can be used in either HI-Control strain.
- Terrific efficiency: The simple, reliable heat-shock procedure for chemical transformation gives excellent results from small amounts of plasmid DNA.
Other Notes
- CONTROLLER BL21(DE3) chemically competent cells
- pUC 19 transformation control DNA
- recovery medium for expression
Classe de stockage
10 - Combustible liquids
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Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| CMC0024-20X40UL | 04061838042569 |