C3306
Chlorure de calcium dihydrate
Molecular Biology, ≥99.0%
Synonyme(s) :
CaCl2.2H2O, Calcium chloride, Calcium dichloride
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A propos de cet article
Formule empirique (notation de Hill) :
CaCl2 · 2H2O
Numéro CAS:
Poids moléculaire :
147.01
NACRES:
NA.31
PubChem Substance ID:
UNSPSC Code:
12352302
EC Number:
233-140-8
MDL number:
Assay:
≥99.0%
Grade:
Molecular Biology
Form:
powder
Solubility:
H2O: 294 mg/mL, clear, colorless
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Molecular Biology
Quality Level
assay
≥99.0%
form
powder
mp
176 °C (dec.) (lit.)
solubility
H2O: 294 mg/mL, clear, colorless
foreign activity
DNase, RNase, protease, none detected
SMILES string
O.O.Cl[Ca]Cl
InChI
1S/Ca.2ClH.2H2O/h;2*1H;2*1H2/q+2;;;;/p-2
InChI key
LLSDKQJKOVVTOJ-UHFFFAOYSA-L
Application
Calcium chloride dihydrate has been used:
to prepare competent E. coli for transformation(118)
in the preparation of calcium polyphosphate nanospheres and to induce alkaline phosphatase(119)
as a component of renaturation buffer for the zymogram of Staphylococcus aureus crude enzyme extracts(120)
to prepare competent E. coli for transformation(118)
in the preparation of calcium polyphosphate nanospheres and to induce alkaline phosphatase(119)
as a component of renaturation buffer for the zymogram of Staphylococcus aureus crude enzyme extracts(120)
Biochem/physiol Actions
Calcium chloride is used in the heat-shock transformation of microbes with the plasmid. The positive charged calcium forms a cation bridge with phosphate of DNA and negative charged phosphorylated lipid A in lipopolysaccharide (LPS) aiding the DNA transformation into the microbes.
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signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Classe de stockage
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Preparation of calcium competent Escherichia coli and heat-shock transformation
Chang AY, et al.
Journal of Experimental Microbiology and Immunology, 1, 22-25 (2017)
Andrew W Moran et al.
Frontiers in nutrition, 5, 101-101 (2018-11-13)
The Na+/glucose cotransporter 1, SGLT1 is the major route for transport of dietary glucose from the lumen of the intestine into absorptive enterocytes. Sensing of dietary sugars and artificial sweeteners by the sweet taste receptor, T1R2-T1R3, expressed in the enteroendocrine
Yujin E Kim et al.
Nature protocols, 5(8), 1396-1405 (2010-07-31)
Applications for noninvasive real-time imaging of apoptosis and neuronal degeneration are hindered by technical limitations in imaging strategies and by existing probes. Monitoring the progression of a cell through apoptosis could provide valuable insight into the temporal events that initiate
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| C3306-500G | 04061833485729 |
| C3306-100G | 04061833485705 |
| C3306-250G | 04061833485712 |