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MilliporeSigma

A8582

Antifoam SE-15

aqueous emulsion for bacterial and mammalian systems

Synonyme(s) :

silicone-based antifoam

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A propos de cet article

NACRES:
NA.85
UNSPSC Code:
12161901
Service technique
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Quality Level

form

emulsion (aqueous)

concentration

10% (active silicon)

technique(s)

cell culture | hybridoma: suitable

solubility

H2O: soluble (effective hot or cold.)

density

1.0 g/mL at 25 °C

application(s)

microbiology

storage temp.

room temp

suitability

(Mammalian (suspension)), bacteria (fermentation)

General description

Antifoam SE-15 is a silicone-based product used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production.

Application

Antifoam SE-15 has been used:

  • in the preparation of ramified rolling circle amplification (RAM) amplification buffer.
  • to find its effects on cell viability, virus infectivity and PCR (polymerase chain reaction) performance.
  • as a constituent of phosphate-buffered saline (PBS) for the determination of total antioxidant status (TAOS) by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay.
  • as a component of autoinduction expression culture.
  • as a component of cyclic adenosine monophosphate (cAMP) detection buffer.

Features and Benefits

  • Controls foaming in microbial culture flasks or fermentations
  • Non-toxic to the culture medium
  • Can be pumped to fermenter on an as-needed basis

Preparation Note

Antifoam SE-15 can be pre-diluted with 3-10 parts of cool water to aid in dispersion. Pre-diluted
suspensions should be used immediately.

Other Notes

Antifoam SE-15 is an aqueous emulsion containing 10% active silicon and non-ionic emulsifiers .


pictograms

Health hazardExclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2 - STOT RE 2 Inhalation

target_organs

Lungs

Classe de stockage

12 - Non Combustible Liquids



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Consulter la Bibliothèque de documents



Dario A Vitturi et al.
American journal of physiology. Heart and circulatory physiology, 296(5), H1398-H1407 (2009-03-17)
Allosteric regulation of nitrite reduction by deoxyhemoglobin has been proposed to mediate nitric oxide (NO) formation during hypoxia. Nitrite is predominantly an anion at physiological pH, raising questions about the mechanism by which it enters the red blood cell (RBC)
Detection of nucleic acid targets using ramified rolling circle DNA amplification: a single nucleotide polymorphism assay model
Smith J H and Beals T P
PLoS ONE, 8(5), e65053-e65053 (2013)
Elisa Binda et al.
BMC biotechnology, 13, 24-24 (2013-03-19)
VanYn, encoded by the dbv7 gene (also known as vanYn) of the biosynthetic cluster devoted to A40926 production, is a novel protein involved in the mechanism of self-resistance in Nonomuraea sp. ATCC 39727. This filamentous actinomycete is an uncommon microorganism



Numéro d'article de commerce international

RéférenceGTIN
A8582-100G04061833392287
A8582-500G04061835572038