A2220
Gel d′affinité ANTI-FLAG® M2
purified immunoglobulin, buffered aqueous glycerol solution
Synonyme(s) :
Anticorps monoclonal ANTI-FLAG® M2 antibody produced in mouse, Gel d’agarose pour chromatographie d’affinité ANTI-FLAG® M2, Anti-ddddk, Anti-dykddddk
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A propos de cet article
conjugate
agarose conjugate
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
M2, monoclonal
form
buffered aqueous glycerol solution
analyte chemical class(es)
proteins
technique(s)
affinity chromatography: suitable, immunoprecipitation (IP): suitable
matrix
(4% agarose bead; 45-165μm bead size)
isotype
IgG1
capacity
>0.6 mg/mL, resin binding capacity (FLAG-BAP)
shipped in
wet ice
storage temp.
−20°C
General description
Élution - peptide FLAG®, glycine, pH 3,5, 3x peptide FLAG®
Immunogen
Application
Pour en savoir plus sur nos produits, rendez-vous sur notre portail des applications des produits FLAG®.
Physical form
Legal Information
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Classe de stockage
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocoles
Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels
Articles
The FLAG® Expression System is a proven method to express, purify and detect recombinant fusion proteins. Sigma®, the proven provider of FLAG®, now offers a magnetic bead for immunoprecipitation, protein purification, and the study of protein-protein interactions. The ANTI-FLAG® M2 Magnetic Bead is composed of murine derived, anti-FLAG® M2 monoclonal antibody attached to superparamagnetic iron impregated 4% agarose beads, with an average diameter of 50 µm. The M2 antibody is capable of binding to fusion proteins containing a FLAG peptide sequence at the N-terminus, Met-N-terminus, or C-terminus locations in mammalian, bacterial, and plant extracts.
Contenu apparenté
Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.
Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| A2220-2X25ML | 04061838089267 |
| A2220-5ML | 04061838251282 |
| A2220-10ML | 04061838061843 |
| A2220-1ML | 04061838061850 |
| A2220-25ML | 04061838251268 |
| A2220-4X25ML | 04061838251275 |