YEAST1
Yeast Transformation Kit
reagents for introducing plasmid DNA into yeast
Synonyme(s) :
lithium acetate yeast transformation
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A propos de cet article
grade
Molecular Biology
Quality Level
usage
kit sufficient for >100 standard transformations
technique(s)
transformation: suitable
shipped in
dry ice
storage temp.
−20°C
General description
Application
Biochem/physiol Actions
Features and Benefits
- Easy and ready-to-use
- Requires as little as 10 ng of plasmid DNA
- Flexibility for any strain of yeast
- Sufficient for over 100 standard transformations
Other Notes
- Transformation Buffer; 100 ml; 100 mM lithium acetate, 10 mM Tris HCl, pH 7.6, and 1 mM EDTA
- Plate Buffer; 100 ml; 40% PEG, 100 mM lithium acetate, 10 mM Tris HCl, pH 7.5, 1 mM EDTA
- Deoxyribonucleic acid from salmon teste, 10 mg/ml; 2 x 1 ml
- Control Yeast Plasmid DNA pRS316 carrying the ura gene; 10 μg
- Yeast Synthetic Drop-out Medium Supplement Without Uracil; 1 g
Classe de stockage
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
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Protocoles
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine, or pyrimidine)
Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.
Articles
Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.
Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.
Contenu apparenté
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| YEAST1-1KT | 04061837588167 |